内质网
荧光
未折叠蛋白反应
化学
生物物理学
内生
调解人
绿色荧光蛋白
分子内力
细胞生物学
生物化学
立体化学
生物
量子力学
基因
物理
作者
Yunyan Zhang,Yonghe Tang,Xiuqi Kong,Weiying Lin
标识
DOI:10.1016/j.saa.2020.119150
摘要
Carbon monoxide (CO) is a significant mediator in regulating endoplasmic reticulum (ER) stress, and its level may play a potential role in the treatment of vascular diseases combined with ER stress. In-situ visualization of CO in the ER helps to elucidate its physiological and pathological mechanistic behavior. Herein, a novel CO fluorescent probe (Na-CM-ER) with ER-targeting characteristics was structured. Na-CM-ER with naphthalimide as a fluorescent group, under the trigger of CO, an ICT (Intramolecular Charge Transfer) mechanism was constructed by converting a nitro group to an amino group and showed dazzling green fluorescence. Na-CM-ER exhibited satisfactory response speed, selectivity, photo-stability and sensitivity to CO in vitro. Furthermore, biological imaging experiments demonstrated that Na-CM-ER could monitor the changes of exogenous/endogenous CO in living cells and possess an ER-targeting property. To sum up, we hope that Na-CM-ER can be as a serviceable molecular tool for imaging CO in cellular ER.
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