诱导多能干细胞
肝细胞
药代动力学
人肝
干细胞
肝功能
生物人工肝装置
细胞
细胞培养
药理学
肝细胞
细胞生物学
功能(生物学)
化学
生物
医学
生物化学
体外
内科学
胚胎干细胞
基因
遗传学
作者
Tomoaki Inoue,Norihiko Iwazaki,Tetsuro Araki,Hiroko Hitotsumachi
出处
期刊:Current Pharmaceutical Biotechnology
[Bentham Science]
日期:2020-06-09
卷期号:21 (9): 773-779
被引量:7
标识
DOI:10.2174/1389201021666200131123524
摘要
Human hepatocytes are essential cell types for pharmacokinetics and the safety evaluation of pharmaceuticals. However, widely used primary hepatocytes with individual variations in liver function lose those functions rapidly in culture. Hepatic cell lines are convenient to use but have low liver functions. Human-Induced Pluripotent Stem (hiPS) cells can be expanded and potentially differentiated into any cell or tissue, including the liver. HiPS cell-derived Hepatocyte-Like Cells (hiPSHeps) are expected to be extensively used as consistent functional human hepatocytes. Many laboratories are investigating methods of using hiPS cells to differentiate hepatocytes, but the derived cells still have immature liver functions. In this paper, we describe the current uses and limitations of conventional hepatic cells, evaluating the suitability of hiPS-Heps to pharmacokinetics and the safety evaluation of pharmaceuticals, and discuss the potential future use of non-conventional non-monolayer culture methods to derive fully functional hiPS-Heps.
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