Characterization of a Kidney Antigen Defined by a Mouse Monoclonal Antibody K2.7

No AccessJournal of Urology1 Jan 1987Characterization of a Kidney Antigen Defined by a Mouse Monoclonal Antibody K2.7 Toshiaki Kinouchi, Eiichi Nakayama, Ryuzo Ueda, Shingo Ishiguro, Akiko Uenaka, Hideaki Oda, and Toshihiko Kotake Toshiaki KinouchiToshiaki Kinouchi More articles by this author , Eiichi NakayamaEiichi Nakayama More articles by this author , Ryuzo UedaRyuzo Ueda More articles by this author , Shingo IshiguroShingo Ishiguro More articles by this author , Akiko UenakaAkiko Uenaka More articles by this author , Hideaki OdaHideaki Oda More articles by this author , and Toshihiko KotakeToshihiko Kotake More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)43908-5AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Abstract: Analysis by indirect immunoperoxidase staining of tissues demonstrated that monoclonal antibody (mAb) K2.7 derived from a mouse immunized with a renal cancer cell line OS-RC-2 was reactive with the tissues of all 36 normal kidneys examined, including two fetal kidneys, 22 of 25 renal cell carcinomas (RCCs) and one of 15 gastric cancers, but not with 23 normal tissues from 11 different organs or 41 malignant tissues from 10 different organs. mAb K2.7 was reactive with the kidneys of several animal species. Positive staining was seen on tubular epithelial cells of the kidney. Thus, the antigen recognized by mAb K2.7 appeared to be an interspecies kidney specific antigen. Results obtained by protein A (PA) assays with a cultured cell panel were consistent with those of staining and confirmed the restricted expression of the antigen. Immunochemical analysis revealed that mAb K2.7 reacted with molecules of 63,000, 60,000 and 43,000 daltons. © 1987 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByKinouchi T, Bander N and Kotake T (2018) Reactivities of Mouse Monoclonal Antibody K2.7 to Renal Cancers in Complement Dependent Cytotoxicity and Antibody Dependent Cell-Mediated CytotoxicityJournal of Urology, VOL. 154, NO. 1, (288-292), Online publication date: 1-Jul-1995. Volume 137Issue 1January 1987Page: 151-154 Advertisement Copyright & Permissions© 1987 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Toshiaki Kinouchi More articles by this author Eiichi Nakayama More articles by this author Ryuzo Ueda More articles by this author Shingo Ishiguro More articles by this author Akiko Uenaka More articles by this author Hideaki Oda More articles by this author Toshihiko Kotake More articles by this author Expand All Advertisement PDF DownloadLoading ...