细胞保护
基因亚型
米诺环素
电压依赖性阴离子通道
酿酒酵母
细胞生物学
酵母
线粒体
细胞
胞浆
化学
生物
细胞凋亡
生物化学
基因
酶
细菌外膜
抗生素
大肠杆菌
作者
Andonis Karachitos,Daria Grobys,Monika Antoniewicz,Sylwia Jedut,Joaquı́n Jordán,Hanna Kmita
出处
期刊:Mitochondrion
[Elsevier]
日期:2016-05-01
卷期号:28: 38-48
被引量:12
标识
DOI:10.1016/j.mito.2016.03.004
摘要
It has been previously demonstrated that cytoprotective activity displayed by minocycline in the case of the yeast Saccharomyces cerevisiae cells pretreated with H2O2 requires the presence of functional VDAC (YVDAC1). Thus, we decided to transform YVDAC1-depleted yeast cells (Δpor1 cells) with plasmids expressing human VDAC isoforms (HVDAC1, HVDAC2, HVDAC3) to estimate their involvement in the minocycline cytoprotective effect. We observed that only expression of HVDAC3 in Δpor1 cells provided minocycline-mediated cytoprotection against H2O2 although all human isoforms are functional in Δpor1 cells. The observation appears to be important for on-going discussion concerning VDAC isoform roles in mitochondria and cell functioning.
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