配体(生物化学)
蛋白质结晶
化学
结晶
辅因子
核酸
分子
结晶学
生物化学
酶
受体
有机化学
作者
Anne M. Hassell,Gang An,Randy K. Bledsoe,Jane Bynum,H. Luke Carter,Su-Jun Deng,Robert T. Gampe,Tamara E. Grisard,Kevin P. Madauss,Robert T. Nolte,Warren J. Rocque,Liping Wang,Kurt Weaver,Shawn P. Williams,G. Bruce Wisely,Renfeng Xu,Lisa M. Shewchuk
出处
期刊:Acta Crystallographica Section D-biological Crystallography
[International Union of Crystallography]
日期:2006-12-13
卷期号:63 (1): 72-79
被引量:128
标识
DOI:10.1107/s0907444906047020
摘要
Obtaining diffraction-quality crystals has long been a bottleneck in solving the three-dimensional structures of proteins. Often proteins may be stabilized when they are complexed with a substrate, nucleic acid, cofactor or small molecule. These ligands, on the other hand, have the potential to induce significant conformational changes to the protein and ab initio screening may be required to find a new crystal form. This paper presents an overview of strategies in the following areas for obtaining crystals of protein-ligand complexes: (i) co-expression of the protein with the ligands of interest, (ii) use of the ligands during protein purification, (iii) cocrystallization and (iv) soaks.
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