Three decades of advances in extraction and analytical techniques for guava (Psidium guajava L.): A review

Despite extensive experimental focus, there remains a notable gap in comprehending the progressive development of these techniques over time. This comprehensive review is dedicated to cataloging the evolution of extraction and analytical methods spanning several decades. In this article, we meticulously traced the development of analytical and extraction techniques. Additionally, we intricately map the periodic discovery of metabolites within guava fruit, aligning them with the chronological utilization of analytical methods. This article results from extensive research and literature analysis to bridge the knowledge gap. Our efforts highlight the advantageous phytochemicals in guava and the diverse extraction techniques available. By encompassing various facets of analytical methods and delving into metabolomics, this review can offer substantial benefits to cultivating future guava varieties. Subsequent sections within this review will serve as a valuable resource, aiding prospective researchers in identifying the most effective extraction and analytical methods for discovering novel metabolites in guava or similar biological samples. Furthermore, through a comprehensive timeline detailing methodological advancements, this review enhances our understanding of the evolutionary path of guava research. Such insights empower researchers to make informed decisions and actively contribute to the continual advancement of the field of research.