Assessment of the mechanistic role of an Indian traditionally used ayurvedic herb Bacopa monnieri (L.)Wettst. In ameliorating oxidative stress in neuronal cells

巴科帕蒙尼里酒店 氧化应激 药理学 神经保护 背景(考古学) 传统医学 嗜中性 医学 生物 生物化学 古生物学
作者
Souvik Ghosh,Viney Kumar,Haimanti Mukherjee,Saakshi Saini,Sumeet Gupta,Samrat Chauhan,Komal Kushwaha,Debrupa Lahiri,Debabrata Sircar,Partha Roy
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:328: 117899-117899
标识
DOI:10.1016/j.jep.2024.117899
摘要

This study has important ethnopharmacological implications since it systematically investigated the therapeutic potential of Bacopa monnieri (L.) Wettst. (Brahmi) in treating neurological disorders characterized by oxidative stress—a growing issue in the aging population. Bacopa monnieri, which is strongly rooted in Ayurveda, has long been recognized for its neuroprotective and cognitive advantages. The study goes beyond conventional wisdom by delving into the molecular complexities of Bacopa monnieri, particularly its active ingredient, Bacoside-A, in countering oxidative stress. The study adds to the ethnopharmacological foundation for using this herbal remedy in the context of neurodegenerative disorders by unravelling the scientific underpinnings of Bacopa monnieri's effectiveness, particularly at the molecular level, against brain damage and related conditions influenced by oxidative stress. This dual approach, which bridges traditional wisdom and modern investigation, highlights Bacopa monnieri's potential as a helpful natural remedy for oxidative stress-related neurological diseases. The aim of this study is to investigate the detailed molecular mechanism of action (in vitro, in silico and in vivo) of Bacopa monnieri (L.) Wettst. methanolic extract and its active compound, Bacoside-A, against oxidative stress in neurodegenerative disorders. ROS generation activity, mitochondrial membrane potential, calcium deposition and apoptosis were studied through DCFDA, Rhodamine-123, FURA-2 AM and AO/EtBr staining respectively. In silico study to check the effect of Bacoside-A on the Nrf-2 and Keap1 axis was performed through molecular docking study and validated experimentally through immunofluorescence co-localization study. In vivo antioxidant activity of Bacopa monnieri extract was assessed by screening the oxidative stress markers and stress-inducing hormone levels as well as through histopathological analysis of tissues. The key outcome of this study is that the methanolic extract of Bacopa monnieri (BME) and its active component, Bacoside-A, protect against oxidative stress in neurodegenerative diseases. At 100 μg/ml, BME and Bacoside-A quenched ROS, preserved mitochondrial membrane potential, decreased calcium deposition, and inhibited HT-22 mouse hippocampus cell death. BME and Bacoside-A regulate the Keap1 and Nrf-2 axis and their downstream antioxidant enzyme-specific genes to modify cellular antioxidant machinery. In vivo experiments utilizing rats subjected to restricted stress indicated that pre-treatment with BME (50 mg/kg) downregulated oxidative stress markers and stress-inducing hormones, and histological staining demonstrated that BME protected the neuronal cells of the Cornu Ammonis (CA1) area in the hippocampus. Overall, the study suggests that Bacopa monnieri (L.) Wettst. has significant potential as a natural remedy for neurodegenerative disorders, and its active compounds could be developed as new drugs for the prevention and treatment of oxidative stress-related diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
xixixi完成签到,获得积分10
1秒前
Franky发布了新的文献求助10
1秒前
冬天雪山茶完成签到,获得积分10
1秒前
yyydd发布了新的文献求助10
2秒前
2秒前
YU发布了新的文献求助10
3秒前
安宇发布了新的文献求助10
3秒前
Azusa完成签到,获得积分10
4秒前
4秒前
李健的小迷弟应助flash_tt采纳,获得10
5秒前
5秒前
CyrusSo524应助明明明采纳,获得10
6秒前
6秒前
元节发布了新的文献求助20
6秒前
7秒前
科研通AI5应助乐观的莫茗采纳,获得30
7秒前
yyydd完成签到,获得积分20
8秒前
crystal发布了新的文献求助10
8秒前
Franky完成签到,获得积分10
8秒前
量子星尘发布了新的文献求助10
8秒前
9秒前
9秒前
倪晨发布了新的文献求助10
9秒前
rubbertail发布了新的文献求助10
9秒前
10秒前
10秒前
10秒前
XIAOWANG完成签到,获得积分10
11秒前
Dr.Lee完成签到 ,获得积分10
11秒前
11秒前
VitoLi发布了新的文献求助10
12秒前
12秒前
Pretext完成签到 ,获得积分10
12秒前
12秒前
落落发布了新的文献求助10
14秒前
研友_VZG7GZ应助yyc采纳,获得10
14秒前
pcr163应助慢行采纳,获得100
15秒前
nannan发布了新的文献求助10
15秒前
15秒前
16秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3970172
求助须知:如何正确求助?哪些是违规求助? 3514982
关于积分的说明 11176568
捐赠科研通 3250212
什么是DOI,文献DOI怎么找? 1795198
邀请新用户注册赠送积分活动 875702
科研通“疑难数据库(出版商)”最低求助积分说明 805004