去细胞化
细胞外基质
材料科学
球体
移植
生物医学工程
脊髓损伤
再生医学
基质(化学分析)
脊髓
组织工程
医学
神经科学
干细胞
细胞生物学
外科
生物
复合材料
体外
生物化学
作者
Kexin Zhang,Zilong Rao,Huiying Zuo,Hanyu Chu,Jiaxin Chen,Rui Cui,Shengwen Zhu,Xiaodong Guo,Yong Hu,Daping Quan,Ying Bai
标识
DOI:10.1002/adfm.202407097
摘要
Abstract Traumatic spinal cord injury (SCI) causes massive death of neurons in the spinal cords and almost complete neurological dysfunctions. Transplantation of neural stem/progenitor cells (NSPCs) is acknowledged as one of the viable SCI treatments for complementing lost neurons and neural network reconstruction. However, administration of NSPCs suffers from extremely low survival rate and uncontrolled differentiation of the transplanted cells, which impairs the therapeutic effects significantly. Herein, NSPCs are encapsulated in decellularized spinal cord matrix (DSCM) microgels using a customized microfluidic system, then the obtained NSPCs‐encapsulated DSCM microgels (NSPC@DSCM‐MGs) are subjected to neuronal differentiation induction. Consequently, the resulting pre‐mature 3D neural spheroids are injected into severely contused spinal cords in rats. The DSCM microgels effectively protected the transplanted cells from shear damage and the inflammatory microenvironment at the lesion site. The survival and accommodation of the pre‐differentiated NSPC@DSCM‐MGs actively contributed to axonal regeneration, inhibiting glial scar formation, as well as remodeling the microenvironment that facilitated endogenous cell recruitment and neuronal network reconstruction. Consequentially, administration of the neural spheroids led to maintenance of spinal cord integrity, and significantly improved hindlimb locomotor function. This biomaterial‐based transplantation strategy has shown unique assets in cell protection and cell‐fate manipulation, which holds great promise in versatile biomedical applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI