SERS‐Encoded Nanoprobes Based on Silver‐Coated Gold Nanorods for Cell Sorting

Abstract Optically‐encoded probes have great potential for applications in the fields of biosensing and imaging. By employing specific encoding methods, these probes enable the detection of multiple target molecules and high‐resolution imaging within the same sample. Among the various encoding methods, surface‐enhanced Raman scattering (SERS) spectral encoding stands out due to its extremely narrow linewidth. Compared to fluorescence spectral encoding, SERS encoding significantly reduces crosstalk between adjacent peaks, thereby achieving a larger encoding capacity and enabling multi‐channel parallel analysis. This article presents the design and construction of two novel sets of SERS‐encoded probes based on noble metal core–shell nanostructures. Two different encoding strategies are successfully applied to encode the SERS spectra of the probes: 1D encoding based on the wavenumber of characteristic peaks in the SERS spectrum, and 2D encoding combining both wavenumber and intensity of characteristic peaks in the SERS spectrum. In addition, this study also demonstrates the potential application of 1D encoded probes in cell sorting. These studies verify the feasibility of applying these two encoding methods to SERS core–shell probes and provide new insights into the construction of optically encoded probes.