Background: Subglottic suction in the intensive care unit (ICU) potentially improve the outcomes of patients need mechanical ventilator (MV) more than 48 hours.Aim: This study aimed to assess nurse's performance regarding subglottic suction.Design: A descriptive explorative design was utilized for the conduction of this study.Setting the study was carried out in intensive care unit (ICU) of Beni-Suef University Hospital.The sample of the study.A convenient sample of 35 nurse.Tools for Data collection.Tool (I) Nurses' Self-administered Questionnaire: Tool (II) Nurses' practice observational Checklist (Appendix II).Results: The main results revealed that: mean age of nurses under study was 28.3±7.33,while 65.7% of the studied nurses were male and 71.4% of them were Technical institute nursing graduates and revealed that,0 % of the studied nurse's had satisfactory level of knowledge about subglottic suction and 2.9of the studied nurses had satisfactory level of practice about subglottic suction.Positive correlation between nurses knowledge, practice and demographic data of nurses under study.Conclusion, based on the findings of the current study, it can be concluded that majority of the nurses were having an unsatisfactory (knowledge, and practice) regarding subglottic suction, and positive correlation between nurses knowledge, practice and demographic data of nurses under study which supported the research question.Recommendations: Develop and implement an educational program based on nurses' learning needs.Assess patients' for ventilator associated pneumonia (VAP) after implementation of the educational program.