溶酶体
化学
程序性细胞死亡
细胞生物学
细胞内
细胞
荧光
细胞器
细胞凋亡
电池类型
生物化学
生物
酶
物理
量子力学
作者
Yaya Wang,Ruiyi Zhao,Haixia Zhang,Hong Gao,Can Gong,Xiaoyan Liu,Haixia Zhang
标识
DOI:10.1021/acs.analchem.2c02036
摘要
Cell death plays a vital role in body development, maintenance of tissue function, and homeostasis. Accurate evaluation of cell death types is of great importance for pharmacological and pathological research. However, there is a lack of efficient fluorescent probes to discriminate various cell states. Here, we design and synthesize a novel activatable fluorescent probe PNE-Lyso to detect intracellular pH and hexosaminidases with two kinds of fluorescence signals. PNE-Lyso could distinguish dead cells from healthy cells based on a dual-color mode by targeting the lysosome and evaluating lysosomal hexosaminidase activity. Significantly, PNE-Lyso could also discriminate apoptotic and necrotic cells through visualizing lysosome morphology that is adjusted by the integrity of the lysosome membrane. Moreover, probe PNE-Lyso was successfully applied to investigate the drug-induced cell death process. To the best of our knowledge, this work is the first time cell death types have been distinguished based on a single fluorescent probe.
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