Bioassay for monitoring the anti-aging effect of cord blood treatment

脐带 代谢物 医学 加速老化 药理学 生物信息学 化学 生物 内科学 免疫学 物理化学
作者
Sang‐Hun Bae,Ala Jo,Jae Hyeon Park,Chul-Woo Lim,Yuri Choi,Juhyun Oh,Ji-Min Park,TaeHo Kong,Ralph Weissleder,Hakho Lee,Jisook Moon
出处
期刊:Theranostics [Ivyspring International Publisher]
卷期号:9 (1): 1-10 被引量:8
标识
DOI:10.7150/thno.30422
摘要

Background: Treating aged animals with plasma of an early developmental stage (e.g, umbilical cord plasma) showed an impressive potential to slow age-associated degradation of neuronal and cognitive functions.Translating such findings to clinical realities, however, requires effective ways for assessing treatment efficacy; ideal methods should be minimally invasive, amenable for serial assays, cost-effective, and quantitative.Methods: We developed a new biosensor approach to monitor anti-aging therapy.We advanced two key sensor components: i) a blood-borne metabolite was identified as a surrogate aging-marker; and ii) a compact and cost-effective assay system was developed for on-site applications.We treated aged mice either with human umbilical cord plasma or saline; unbiased metabolite profiling on mouse plasma revealed arachidonic acid (AA) as a potent indicator associated with anti-aging effect.We next implemented a competitive magneto-electrochemical sensor (cMES) optimized for AA detection directly from plasma.The developed platform could detect AA directly from small volumes of plasma (0.5 µL) within 1.5 hour.Results: cMES assays confirmed a strong correlation between AA levels and anti-aging effect: AA levels, while decreasing with aging, increased in the plasma-treated aged mice which also showed improved learning and memory performance.Conclusions: The cMES platform will empower both pre-and clinical anti-aging research by enabling minimally invasive, longitudinal treatment surveillance; these capacities will accelerate the development of anti-aging therapies, improving the quality of individual lives.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
hjkl发布了新的文献求助10
2秒前
zzzlll发布了新的文献求助20
2秒前
东方立轩发布了新的文献求助10
2秒前
hgm完成签到 ,获得积分10
2秒前
顾矜应助杰森斯坦虎采纳,获得10
3秒前
6秒前
wwdd完成签到,获得积分10
7秒前
爱睡觉的修勾勾完成签到,获得积分20
7秒前
不配.应助彪壮的绮烟采纳,获得10
9秒前
9秒前
9秒前
planA完成签到,获得积分10
9秒前
10秒前
acd完成签到,获得积分10
11秒前
11秒前
张文完成签到,获得积分10
11秒前
sdkabdrxt发布了新的文献求助10
12秒前
李健应助pysa采纳,获得10
12秒前
14秒前
xiaoxiao发布了新的文献求助10
14秒前
Hello应助咿呀咿呀哟采纳,获得10
15秒前
16秒前
江大橘完成签到,获得积分10
16秒前
17秒前
17秒前
萌主发布了新的文献求助10
17秒前
replica完成签到,获得积分10
17秒前
陈龙发布了新的文献求助10
18秒前
酷波er应助Sky36001采纳,获得10
19秒前
20秒前
fzzzzlucy发布了新的文献求助10
20秒前
21秒前
22秒前
LYDZ2完成签到,获得积分10
23秒前
勤劳的傲晴完成签到,获得积分10
23秒前
24秒前
kb发布了新的文献求助10
26秒前
tt完成签到,获得积分10
28秒前
小马甲应助微糖煎蛋采纳,获得10
28秒前
柚子完成签到,获得积分10
29秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3182979
求助须知:如何正确求助?哪些是违规求助? 2833161
关于积分的说明 7992824
捐赠科研通 2495326
什么是DOI,文献DOI怎么找? 1331455
科研通“疑难数据库(出版商)”最低求助积分说明 636293
邀请新用户注册赠送积分活动 603391