Bioassay for monitoring the anti-aging effect of cord blood treatment

脐带 代谢物 医学 加速老化 药理学 生物信息学 化学 生物 内科学 免疫学 物理化学
作者
Sang‐Hun Bae,Ala Jo,Jae Hyeon Park,Chul-Woo Lim,Yuri Choi,Juhyun Oh,Ji-Min Park,TaeHo Kong,Ralph Weissleder,Hakho Lee,Jisook Moon
出处
期刊:Theranostics [Ivyspring International Publisher]
卷期号:9 (1): 1-10 被引量:8
标识
DOI:10.7150/thno.30422
摘要

Background: Treating aged animals with plasma of an early developmental stage (e.g, umbilical cord plasma) showed an impressive potential to slow age-associated degradation of neuronal and cognitive functions.Translating such findings to clinical realities, however, requires effective ways for assessing treatment efficacy; ideal methods should be minimally invasive, amenable for serial assays, cost-effective, and quantitative.Methods: We developed a new biosensor approach to monitor anti-aging therapy.We advanced two key sensor components: i) a blood-borne metabolite was identified as a surrogate aging-marker; and ii) a compact and cost-effective assay system was developed for on-site applications.We treated aged mice either with human umbilical cord plasma or saline; unbiased metabolite profiling on mouse plasma revealed arachidonic acid (AA) as a potent indicator associated with anti-aging effect.We next implemented a competitive magneto-electrochemical sensor (cMES) optimized for AA detection directly from plasma.The developed platform could detect AA directly from small volumes of plasma (0.5 µL) within 1.5 hour.Results: cMES assays confirmed a strong correlation between AA levels and anti-aging effect: AA levels, while decreasing with aging, increased in the plasma-treated aged mice which also showed improved learning and memory performance.Conclusions: The cMES platform will empower both pre-and clinical anti-aging research by enabling minimally invasive, longitudinal treatment surveillance; these capacities will accelerate the development of anti-aging therapies, improving the quality of individual lives.

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