胸苷
细胞周期
小区同步
DNA复制
DNA合成
细胞生物学
同步(交流)
细胞
人口
DNA
生物
化学
分子生物学
计算机科学
遗传学
医学
计算机网络
环境卫生
频道(广播)
作者
Guo Chen,Xingming Deng
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2018-01-01
卷期号:8 (17)
被引量:132
标识
DOI:10.21769/bioprotoc.2994
摘要
Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at G1/S boundary by using double thymidine block. After release into normal medium, cell population at distinct cell cycle phase could be collected at different time points.
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