化学
荧光
罗丹明B
检出限
壳聚糖
荧光光谱法
肝素
石墨烯
抗坏血酸
光化学
分析化学(期刊)
核化学
色谱法
生物化学
材料科学
纳米技术
光催化
物理
量子力学
催化作用
食品科学
作者
Linlin Zhong,Kyusik Yun
出处
期刊:Methods and Applications in Fluorescence
[IOP Publishing]
日期:2018-05-16
卷期号:6 (3): 035011-035011
被引量:7
标识
DOI:10.1088/2050-6120/aac51c
摘要
A novel fluorescence 'Switch on' for the detection of heparin based on the RhB-COL/GO system was achieved. A strong fluorescence dye, Rhodamine B, was modified by chitosan oligosaccharide lactate (COL), which plays a major role in the formation of a positively charged RhB-COL complex. RhB-COL was soluble and stable in solution, which was characterized by using Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy. GO sheets quenched the fluorescence intensity of RhB-COL due to electron transfer from RhB to the GO surface. The decrease in fluorescence intensity of RhB-COL with increasing GO concentration was recorded using a Cary Eclipse fluorescence spectrophotometer. On the other hand, the addition of heparin replaced GO to bind with the RhB-COL surface via an electrostatic and noncovalent bond due to the abundant negative charge, which resulted in recovery of the fluorescence intensity. This RhB-COL/GO system possessed high selectivity and good sensitivity for the detection of heparin compared to other biomolecules, such as glycine, D-glucose, hyaluronic acid, L-glutamic acid, and ascorbic acid. The linear response toward heparin was measured over the range, 0-1.8 U · ml-1, with a low detection limit of 0.04 U · ml-1. The satisfactory sensing performance of RhB-COL/GO for heparin supports new 'switch-on' sensor applications in heparin-related biomedical detection.
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