适体
化学
安培法
组合化学
检出限
寡核苷酸
谷氨酸受体
生物传感器
生物物理学
电极
电化学
生物化学
色谱法
DNA
生物
分子生物学
物理化学
受体
作者
Changtong Wu,Daria Barkova,Н. В. Комарова,Andreas Offenhäusser,Mariia Andrianova,Ziheng Hu,Alexander Kuznetsov,Dirk Mayer
标识
DOI:10.1007/s00216-021-03783-w
摘要
An electrochemical aptamer-based sensor was developed for glutamate, the major excitatory neurotransmitter in the central nervous system. Determining glutamic acid release and glutamic acid levels is crucial for studying signal transmission and for diagnosing pathological conditions in the brain. Glutamic acid-selective oligonucleotides were isolated from an ssDNA library using the Capture-SELEX protocol in complex medium. The selection permitted the isolation of an aptamer 1d04 with a dissociation constant of 12 µM. The aptamer sequence was further used in the development of an electrochemical aptamer sensor. For this purpose, a truncated aptamer sequence named glu1 was labelled with a ferrocene redox tag at the 3'-end and immobilized on a gold electrode surface via Au-thiol bonds. Using 6-mercapto-1-hexanol as the backfill, the sensor performance was characterized by alternating current voltammetry. The glu1 aptasensor showed a limit of detection of 0.0013 pM, a wide detection range between 0.01 pM and 1 nM, and good selectivity for glutamate in tenfold diluted human serum. With this enzyme-free aptasensor, the highly selective and sensitive detection of glutamate was demonstrated, which possesses great potential for implementation in microelectrodes and for in vitro as well as in vivo monitoring of neurotransmitter release.
科研通智能强力驱动
Strongly Powered by AbleSci AI