流式细胞术
质量细胞仪
黑色素瘤
细胞
细胞质
核心
癌症研究
生物
细胞仪
细胞生物学
免疫系统
化学
分子生物学
免疫学
基因
生物化学
表型
作者
Daniela Tantalo,Thu Nguyen,Han Xian Aw Yeang,Joe Jiang Zhu,Sean Macdonald,Minyu Wang,Harini de Silva,Criselle D’Souza,Angela Pizzolla,Paul J. Neeson
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 543-555
被引量:2
标识
DOI:10.1007/978-1-0716-1205-7_38
摘要
Here we describe the application of mass cytometry to analyze tumor-infiltrating lymphocytes in human melanoma. Mass cytometry is the coupling of flow cytometry and mass spectrometry, which allows for the simultaneous measurement of 40+ cell parameters on a per cell basis. Heavy metal-labeled antibodies can bind to proteins (CD markers, transcription factors, cytokines) on the cell surface and in the cytoplasm/nucleus. As labeled cells pass through the CyTOF, the instrument detects the heavy metals. Combining these signals allows description of melanoma tumor-infiltrating lymphocytes at a greater depth than alternative phenotyping strategies and enables detailed analyses of a variety of cellular parameters, including immune cell lineage, activation status, and functional polarization.
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