Effect of root exudates on AM colonization in J atropha curcas was studied in the current work. Total three treatments were tested viz., root exudates + AM fungi, AM fungi alone, and root exudates alone. Out of which the treatment containing AM fungi + root exudates showed maximum (75%) colonization followed by AM fungi alone. Impact on plant nutrient status was also observed and significant results were recorded. There was remarkable enhancement in phosphatase enzyme activity, phosphorus uptake and leaf protein in treatment containing AM fungi and root exudates as compared to control. Therefore, from the present findings, it can be concluded that root exudates affect the root colonization of AM fungi which in turn improve the growth and development of biofuel plant J.curcas .