猫抓病
巴尔通氏体
巴尔通体
生物
聚合酶链反应
免疫组织化学
分子生物学
免疫染色
底漆(化妆品)
病理
病毒学
基因
医学
抗体
化学
疾病
血清学
免疫学
遗传学
有机化学
作者
Xiang Qian,Long Jin,Randall T. Hayden,Ellen D. McPhail,Ricardo V. Lloyd
出处
期刊:Diagnostic Molecular Pathology
[Ovid Technologies (Wolters Kluwer)]
日期:2005-08-16
卷期号:14 (3): 146-151
被引量:24
标识
DOI:10.1097/01.pas.0000176771.64165.fb
摘要
Cat scratch disease (CSD) is commonly caused by Bartonella henselae infection. Clinical history and histologic findings are often insufficient to establish a definitive diagnosis of CSD. We retrospectively studied formalin-fixed, paraffin-embedded (FFPE) lymph nodes from 35 patients with histologically suspected CSD by 2 different PCR assays and immunohistochemistry (IHC). The first primer pair amplified a 163-bp fragment of the 16S rRNA gene in 19 of the 35 cases (54%). The second primer pair amplified a 191-bp fragment of the henselae citrate synthase (gltA) gene in 17 of the 35 cases (49%). IHC identified the organisms in 8 of 33 cases (24%). Fresh cultures of various Bartonella species showed a specific PCR product with an analytical sensitivity of 0.5 to 5 pg bacterial DNA. Bartonella species were identified by the unique size of the amplified PCR product. Twenty-two lymph nodes without morphologic evidence or a history of CSD were negative by PCR and immunostaining. Tissues from a patient with Legionella pneumophila were also negative by PCR and immunostaining for CSD supporting the specificity of the PCR reaction. The specific PCR products of the B. henselae were confirmed by sequencing. Human beta-actin for each case was amplified to check the integrity of the DNA. Our data indicate that detection of Bartonella DNA by PCR is useful to confirm the diagnosis of CSD.
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