Surface‐enhanced Raman scattering (SERS) spectra of hemoglobin on nano silver film prepared by electrolysis method

Abstract Nano silver films were prepared by the electrolysis method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to detect the morphology of the silver particles. The surface‐enhanced Raman scattering (SERS) spectra of the hemoglobin on nano silver film were recorded. It is seen from the SERS spectra that the nano silver films can enhance the Raman signals of the hemoglobin efficiently, and sodium citrate and phosphate buffered saline have no influence on the SERS spectra of hemoglobin. The electrolysis technique to fabricate this highly bioactive, stable, reusable, and low‐cost SERS substrate will be useful in the development of hemoglobin detection methods. Copyright © 2009 John Wiley & Sons, Ltd.