间充质干细胞
杰纳斯
海藻酸钙
药物输送
钙
干细胞
生物医学工程
化学
微球
生物物理学
材料科学
纳米技术
化学工程
细胞生物学
病理
医学
有机化学
工程类
生物
作者
Reju George Thomas,Afeesh Rajan Unnithan,Myeong Ju Moon,Suchithra Poilil Surendran,Batgerel Tumurbaatar,Chan Hee Park,Cheol Sang Kim,Yong Yeon Jeong
标识
DOI:10.1016/j.ijbiomac.2018.01.003
摘要
We prepared Janus microspheres based on sodium alginate for the encapsulation of mesenchymal stem cells (MSC) in one compartment and iron oxide nanoparticles (IONP) or a drug in the second compartment. 4% percent sodium alginate solution was allowed to pass through a septum-theta capillary device and react with 2.5% calcium chloride to allow crosslinking to occur in the solution, forming calcium alginate Janus microspheres. Physico-chemical characterization of microspheres was done by FTIR, TGA, and XRD after loading of stem cells and IONP/drug. The mechanical integrity of microspheres was tested at different time points, which showed that 4% alginate microspheres were mechanically stable for a long period of time. Live/dead staining of MSCs alone and the MTS assay of MSCs and DMSO co-loaded were performed, which showed less toxicity to MSC in the Janus configuration. IONP/MSC-loaded Janus microspheres were tested by magnetic manipulation for targeted MSC delivery for cartilage repair using an electromagnetic manipulation (EMM) device. Janus microspheres can be used for targeted stem cell/drug delivery using EMM for cartilage repair in the near future.
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