生物膜
溶解循环
微生物学
噬菌体
噬菌体疗法
细菌
生物
多细胞生物
大肠杆菌
病毒学
细胞
病毒
基因
遗传学
作者
Lucia Vidakovic,Praveen K. Singh,Raimo Hartmann,Carey D. Nadell,Knut Drescher
出处
期刊:Nature microbiology
日期:2017-10-27
卷期号:3 (1): 26-31
被引量:258
标识
DOI:10.1038/s41564-017-0050-1
摘要
In nature, bacteria primarily live in surface-attached, multicellular communities, termed biofilms 1-6 . In medical settings, biofilms cause devastating damage during chronic and acute infections; indeed, bacteria are often viewed as agents of human disease 7 . However, bacteria themselves suffer from diseases, most notably in the form of viral pathogens termed bacteriophages 8-12 , which are the most abundant replicating entities on Earth. Phage-biofilm encounters are undoubtedly common in the environment, but the mechanisms that determine the outcome of these encounters are unknown. Using Escherichia coli biofilms and the lytic phage T7 as models, we discovered that an amyloid fibre network of CsgA (curli polymer) protects biofilms against phage attack via two separate mechanisms. First, collective cell protection results from inhibition of phage transport into the biofilm, which we demonstrate in vivo and in vitro. Second, CsgA fibres protect cells individually by coating their surface and binding phage particles, thereby preventing their attachment to the cell exterior. These insights into biofilm-phage interactions have broad-ranging implications for the design of phage applications in biotechnology, phage therapy and the evolutionary dynamics of phages with their bacterial hosts.
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