Toxicological assessment of cadmium-containing quantum dots in developing zebrafish: Physiological performance and neurobehavioral responses

The present research investigated the effects of exposure to sublethal concentrations of cadmium selenide/zinc sulfide (CdSecore/ZnSshell)-containing quantum dots (QDs; 0 - 100 µg/L QDs) on the neurophysiological performance of developing zebrafish (Danio rerio). The results suggested that exposure to CdSe QDs for 5 days increased the whole-body content of Cd without affecting the general physiological conditions of larvae. Interestingly, CdSe QD exposure reduced swimming distance but increased swimming velocity of larvae, suggesting that the exposure may lead to burst/episodic swimming. The findings also suggested that CdSe QD exposure reduced the wall-hugging behavior of larvae during a sudden light-to-dark transition test, and that the exposure significantly decreased the locomotor activity of fish during the dark period. On the other hand, control larvae displayed a dark avoidance behavior, whereas CdSe QD-exposed larvae exhibited an increase in the time spent in the dark zone, providing further support that CdSe QDs inhibited anxiety-related responses in larvae. Additional analysis with droplet digital PCR revealed that CdSe QD exposure altered the mRNA levels of genes that are associated with dopamine signaling and oxidative stress response. Collectively, our findings suggested that CdSe QD exposure may induce neurobehavioural toxicity and alters the mRNA abundance of dopamine- and oxidative stress-related genes in developing animals.