荧光
聚集诱导发射
纳米技术
猝灭(荧光)
酶
化学
体内
生物物理学
生物化学
材料科学
生物
生物技术
物理
量子力学
作者
Jianguo Wang,Guoyu Jiang
标识
DOI:10.1002/9781119643098.ch54
摘要
Enzymes play vital roles in maintaining normal life activities. Activity abnormalities of specific enzymes are usually associated with the occurrence and the development of many diseases. Thus, they are also very important biomarkers for these diseases. The great importance of enzymes has aroused many researchers' interest to develop highly selective and sensitive methods to monitor their activity/level in vitro and in vivo. Among various detection techniques colorimetric, fluorogenic, chemiluminescent and bioluminescent approaches, fluorescent probes are of great interest for their high sensitivity and selectivity, simple handling procedure, inexpensive instruments, and bioimaging ability. However, most of the reported fluorescent probes for enzymes are fabricated with traditional fluorophores, which suffered from aggregation-caused quenching (ACQ) of fluorescence in high concentration solutions or after they accumulated in cells, making the fluorescent emission much weaker as compared to that in solution. Fortunately, the recently emerged luminogens with aggregation-induced emission (AIE) feature could perfectly overcome the ACQ effect of conventional fluorophores. Based on the fantastic characteristics of AIE luminogens (AIEgens), many fluorescent probes for enzymes have been designed, demonstrating the unique advantages of AIE molecules including low background interference, large Stokes shifts, superior photostability, and so on. In this chapter, we mainly summarize the working principle and research progress of AIE-active fluorescence probes for enzymes, discuss their potential applications in disease theranostics, and explore their future application prospects.
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