神经发生
自愈水凝胶
细胞外基质
再生(生物学)
粘弹性
神经干细胞
材料科学
生物物理学
化学
生物医学工程
细胞生物学
神经科学
干细胞
生物
高分子化学
医学
复合材料
作者
Suping Chen,Chengheng Wu,Ting Zhou,Kai Wu,Nini Xin,Xiaoyin Liu,Zi Qiao,Dan Wei,Jing Sun,Hongrong Luo,Liangxue Zhou,Hongsong Fan
标识
DOI:10.1016/j.carbpol.2021.118961
摘要
Either oriented architecture or viscoelasticity is pivotal to neurogenesis, thus, native neural extracellular matrix derived-hyaluronan hydrogels with nano-orientation and viscoelasticity recapitulated might be instructive for neurogenesis, however it is still unexploited. Herein, based on aldehyde-methacrylate difunctionalized hyaluronan, by integrating imine kinetic modulation and microfluidic biofabrication, we construct a hydrogel system with orthogonal viscoelasticity and nano-topography. We then find the positive synergy effects of matrix nano-orientation and viscoelasticity not only on neurites outgrowth and elongation of neural cells, but also on neuronal differentiation of stem cells. Moreover, by implanting viscoelastic and nano-aligned hydrogels into lesion sites, we demonstrate the enhanced repair of spinal cord injury, including ameliorated pathological microenvironment, facilitated endogenous neurogenesis and functional axons regeneration as well as motor function restoration. This work supplies universal platform for preparing neuronal inducing hyaluronan-based hydrogels which might serve as promising therapeutic strategies for nerve injury.
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