Antibacterial mechanism of sucrose laurate against Bacillus cereus by attacking multiple targets and its application in milk beverage

蜡样芽孢杆菌 抗菌活性 化学 细菌细胞结构 生物化学 细胞壁 碱性磷酸酶 细菌 生物 遗传学
作者
Yawei Ning,Mengge Ma,Qian Zhang,Dongchun Zhang,Linlin Hou,Kun Yang,Yunan Fu,Zhixin Wang,Yingmin Jia
出处
期刊:Food Research International [Elsevier]
卷期号:154: 111018-111018 被引量:20
标识
DOI:10.1016/j.foodres.2022.111018
摘要

Sucrose laurate (SL) is a promising dual-functional additive due to its emulsification and antibacterial activity. However, the knowledge on the antibacterial action of SL against Bacillus cereus was lacking, and thus it was investigated from multiple targets. The antibacterial results demonstrated that the minimum inhibitory concentration of SL was 0.3125 mg/mL, and the time-killing curve confirmed the strong antibacterial activity of SL. The alkaline phosphatase assay suggested that SL disrupted the cell wall integrity. The flow cytometry and fluorescence spectroscopy analysis indicated that SL damaged the integrity of cell membrane and dissipated the transmembrane potential, resulting in the leakage of intracellular materials, which were further supported by scanning electron microscopy and transmission electron microscopy. iTRAQ-based proteomic analysis indicated that SL down-regulated cell wall-associated hydrolase, inhibited the synthesis of fatty acids, influenced nucleic acid synthesis, disturbed amino acid metabolism, and blocked HMP pathway and TCA cycle. Finally, the promising application of SL was evidenced in milk beverage. This investigation could provide scientific basis for the practical application of SL as a dual-functional food additive.
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