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Kaempferol ameliorates in-vitro and in-vivo postovulatory oocyte ageing in mice

山奈酚 卵母细胞 男科 生物 体外成熟 老化 胚胎 细胞生物学 医学 抗氧化剂 生物化学 类黄酮 遗传学
作者
Zhao-Cheng Zeng,Jiang Jiang,Xin-Jie Wang,Kang-Na Wei,Hui-Sheng Liang,Li-Xin Zeng,Ying Xu,Shu‐Juan Xie,Zhe Meng,Xiangjun Yang,Aiwei Guo,Hailong Wang
出处
期刊:Reproductive Biomedicine Online [Elsevier]
卷期号:45 (6): 1065-1083 被引量:7
标识
DOI:10.1016/j.rbmo.2022.07.005
摘要

Does kaempferol alleviate postovulatory oocyte ageing, thereby maintaining their early embryonic development capacity?The effects of kaempferol on postovulatory ageing were investigated in vitro and in vivo by short-term kaempferol administration (mature oocytes were cultured in a kaempferol-containing medium for 12 h; mice were intragastrically administered with the appropriate amount of kaempferol for 21 days). Spindle morphology and chromosome alignment, levels of oxidative stress and the gap junction were assessed by immunofluorescence. Fertilization ability and early embryonic development ability of each oocyte group was detected by IVF. Fertilization of the ageing oocyte model was used to explore whether kaempferol could improve adverse pregnancy outcome. RNA-sequencing and quantitative polymerase chain reaction were used to identify the cellular pathways through which kaempferol relieves postovulatory oocyte ageing in vivo.Kaempferol administration altered various processes in the ageing oocytes, including oxidative stress, the peroxisome, TNF signalling, cAMP signalling and the gap junction pathway. Expression of several important genes, such as Sirt1, Mapk1, Ampk and Foxo3, was regulated. Moreover, kaempferol ameliorated adverse pregnancy outcomes of fertilized ageing oocytes. IVF results indicate that kaempferol could partially counteract the effects of oocyte ageing on fertilization capacity (pronucleus: kaempferol, 69.08 ± 2.37% versus aged, 38.95 ± 3.58%) and early embryonic development (blastocyst: kaempferol, 50.02 ± 3.34% versus aged, 30.83 ± 5.46%).Our results indicate that kaempferol may be a potent natural antioxidant, have implications for animal husbandry and may help improve the success rate of IVF and ICSI. Further clinical trials are needed.
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