Identification of shoot and root genomic regions in response to nitrogen deficiency tolerance in rice through the use of rice back cross recombinant inbred lines (BRILs)

Nitrogen (N) is one of the most important factors that could link crop production and play an important role in the formation of rice cultivation. In this study, we used 117 rice backcross recombinant inbred lines (BRILs) derived from a cross indica cv. Changhui 891 and japonica cv. 02,428. To elucidate the genetic control of N deficiency tolerance in rice. A total of fourteen QTLs were reported under the two N levels and their ratio, with important LOD values for six traits. These 14 QTLs are located on the six chromosomes such as Ch01, Ch02, Ch03, Ch04, Ch09, and Ch11. Only one QTL (qRFW1) from these QTLs has the increasing additive effect, 4 QTLs (qSFW9, qRFW1, qRFW11, and qSDW9) were identified under the normal nitrogen condition (N+), 4 QTLs (qSFW11, qRFW1, qRFW3, and qRFW11) were identified under N deficiency (N−) conditions, and remaining 6 QTLs (qSL4, qRL9, qSFW1, qRFW, qRFW2, and qSDW3) were mapped under N+/N− ratio. Out of the 14 QTLs, qRFW-3 under N− and qSDW-3 under N+/N− ratio are pioneer QTLs. Shoot fresh weight and shoot dry weight were directly related to one another QTLs were placed on the same chromosome 9 for these variables in the analogous region. For further research, including fine mapping and cloning, these results from this study may act as a crucial genetic variation that can be helpful in the breeding of molecular markers.