角膜内皮
角膜移植
移植
间充质干细胞
内皮
医学
内皮功能障碍
上皮-间质转换
失明
体外
失调家庭
体内
免疫学
癌症研究
内皮干细胞
细胞生物学
病理
生物
生物技术
外科
内科学
癌症
验光服务
遗传学
转移
临床心理学
作者
Swatilekha Hazra,Iskala V. Sneha,Sunita Chaurasia,Charanya Ramachandran
出处
期刊:Cornea
[Ovid Technologies (Wolters Kluwer)]
日期:2022-06-22
卷期号:41 (10): 1313-1324
被引量:1
标识
DOI:10.1097/ico.0000000000003080
摘要
Abstract: Endothelial dysfunction is one of the leading causes of corneal blindness and one of the common indications for keratoplasty. At present, the standard of treatment involves the replacement of the dysfunctional endothelium with healthy tissue taken from a donor. Because there is a paucity of healthy donor tissues, research on the corneal endothelium has focused primarily on expanding these cells in the laboratory for transplantation in an attempt to reduce the gap between the demand and supply of donor tissues for transplantation. To expand these cells, which are nonmitotic in vivo, various mitogens, substrates, culture systems, and alternate strategies have been tested with varying success. The biggest challenge has been the limited proliferative capacity of these cells compounded with endothelial to mesenchymal transition that alters the functioning of these cells and renders them unsuitable for human transplantation. This review aims to give a comprehensive overview of the most common and successful techniques used in the culture of the cells, the current available evidence in support of epithelial to mesenchymal transition (EMT), alternate sources for deriving the corneal endothelial cells, and advances made in transplantation of these cells.
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