Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker

柑橘×冬青 抗性(生态学) 转基因作物 芸香科
作者
Hong-Ti Jia,Yunzeng Zhang,Vladimir Orbović,Jin Xu,Frank F. White,Jeffrey B. Jones,Nian Wang
出处
期刊:Plant Biotechnology Journal [Wiley]
卷期号:15 (7): 817-823 被引量:344
标识
DOI:10.1111/pbi.12677
摘要

Abstract Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing ( HLB ). Breeding for disease‐resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR /Cas9/sg RNA technology to modify the canker susceptibility gene Cs LOB 1 in Duncan grapefruit. Six independent lines, D LOB 2, D LOB 3, D LOB 9, D LOB 10, D LOB 11 and D LOB 12, were generated. Targeted next‐generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for D LOB 2, D LOB 3, D LOB 9, D LOB 10, D LOB 11 and D LOB 12, respectively, of the cells in each line. D LOB 2 and D LOB 3 showed canker symptoms similar to wild‐type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on D LOB 9, D LOB 10, D LOB 11 and D LOB 12 at 4 days postinoculation ( DPI ) with Xcc. Pustules caused by Xcc were observed on D LOB 9, D LOB 10, D LOB 11 and D LOB 12 in later stages, which were much reduced compared to that on wild‐type grapefruit. The pustules on D LOB 9 and D LOB 10 did not develop into typical canker symptoms. No side effects and off‐target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease‐resistant citrus varieties.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
YEFEIeee完成签到 ,获得积分10
刚刚
1秒前
dochx完成签到,获得积分10
1秒前
赵焱峥完成签到,获得积分10
2秒前
兔子完成签到,获得积分10
2秒前
lisa发布了新的文献求助10
4秒前
5秒前
默默新儿完成签到 ,获得积分10
5秒前
悠然xz关注了科研通微信公众号
5秒前
aaaaazhou发布了新的文献求助10
5秒前
洁白的故人完成签到 ,获得积分10
7秒前
7秒前
打打应助DNA采纳,获得30
7秒前
Phosphene应助helitrope采纳,获得10
8秒前
单纯大侠发布了新的文献求助10
9秒前
专注的夜蓉完成签到,获得积分10
9秒前
1257应助拔丝香芋采纳,获得10
11秒前
12秒前
12秒前
在水一方应助yu001采纳,获得10
14秒前
HPP123完成签到,获得积分10
14秒前
14秒前
杨大大完成签到,获得积分20
14秒前
14秒前
K先生完成签到,获得积分10
15秒前
haoliu完成签到,获得积分10
15秒前
科目三应助gggja采纳,获得10
16秒前
神勇从波发布了新的文献求助10
17秒前
苗条青槐发布了新的文献求助10
17秒前
小蘑菇应助宋宋采纳,获得10
20秒前
隐形曼青应助拔丝香芋采纳,获得10
21秒前
852应助干煸四季豆采纳,获得10
22秒前
zhaoxi完成签到 ,获得积分10
22秒前
科目三应助liyangyang0816采纳,获得10
22秒前
23秒前
25秒前
lzy发布了新的文献求助10
25秒前
yu001发布了新的文献求助10
26秒前
刘慧鑫完成签到 ,获得积分10
27秒前
高分求助中
Sustainability in ’Tides Chemistry 2000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Threaded Harmony: A Sustainable Approach to Fashion 810
Handbook of the Mammals of the World – Volume 3: Primates 805
Ethnicities: Media, Health, and Coping 800
Gerard de Lairesse : an artist between stage and studio 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3074227
求助须知:如何正确求助?哪些是违规求助? 2727639
关于积分的说明 7499897
捐赠科研通 2375736
什么是DOI,文献DOI怎么找? 1259523
科研通“疑难数据库(出版商)”最低求助积分说明 610724
版权声明 597081