生物化学
辅酶A
羟基肉桂酸
肉桂醇脱氢酶
咖啡酸
代谢物
生物
生物合成
激发子
化学
O-甲基转移酶
苯丙氨酸
苯丙氨酸解氨酶
绿原酸
基因表达
甲基转移酶
酶
基因
单甘醇
苯丙素
类黄酮生物合成
植保素
氨基酸
还原酶
抗氧化剂
过氧化物酶
甲基化
作者
Weiting Ni,Vincent Sewalt,Kenneth L. Korth,Jack W. Blount,G. M. Ballance,Richard A. Dixon
出处
期刊:Plant Physiology
[Oxford University Press]
日期:1996-10-01
卷期号:112 (2): 717-726
被引量:29
摘要
Transcription of genes encoding L-phenylalanine ammonia-lyase (PAL), the first enzyme of the phenylpropanoid pathway, and caffeic acid 3-O-methyltransferase (COMT) and caffeoyl CoA 3-O-methyltransferase (CCOMT), enzymes involved in the synthesis of lignin and wall-esterified phenolic compounds, was strongly activated in elicitor-treated cell-suspension cultures of alfalfa (Medicago sativa L.). However, consequent changes in the extractable activities of COMT and CCOMT were small to nonexistent compared with a 15- to 16-fold increase in PAL activity. Only low levels of COMT and CCOMT transcripts were reflected in the total and polysomal RNA fractions compared with PAL transcripts. Elicited cell cultures did not accumulate lignin or the products of COMT and CCOMT in the soluble and wall-esterified phenolic fractions. In one alfalfa cell line in which elicitation resulted in very high PAL activity and increased deposition of methoxyl groups in the insoluble wall fraction, there was still no change in COMT and CCOMT activities. Overall, these results indicate that the initial gene transcription events in elicited cells may be less selective than the subsequent metabolic changes, highlighting the importance of posttranscriptional events in the control of phenylpropanoid biosynthesis.
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