Comparison of three sperm preparation media.

Percoll公司 精子 男科 精液 运动性 精子活力 电容 生物 人类受精 精液分析 解剖 离心 医学 不育 生物化学 细胞生物学 怀孕 遗传学
作者
Rakesh Sharma,Karen Seifarth,D Garlak,Ashok Agarwal
出处
期刊:PubMed 卷期号:44 (3): 163-7 被引量:7
链接
标识
摘要

In assisted reproduction, spermatozoa must be effectively separated from seminal plasma to undergo capacitation, a prerequisite for fertilization. Percoll was recently withdrawn from the American market because of safety concerns. The purpose of this study was to evaluate the quality of sperm separated by two different sperm washing media, and compare these results to sperm quality after separation on a Percoll (Perwash) gradient.Semen samples from 10 normozoospermic men were compared after separation on three media: Perwash, a Percoll-type medium (Conception Technologies, La Jolla, CA), ISolate (Irvine Scientific, Santa Ana, CA), and SpermFertil (Embryotech Laboratories, Wilmington, MA). Semen characteristics examined were: sperm count, percentage motility, curvilinear velocity, lateral head displacement, percentage recovery of motile sperm, viability, hypo-osmotic swelling, and penetration in bovine cervical mucus. Sperm morphology was scored using World Health Organization and Kruger's strict criteria. The motility of the sperm was examined at one-hour intervals for three hours to determine which method produced samples with the longest period of motility.Total motile sperm count, motility, curvilinear velocity, and percentage of normal morphological forms as determined by the WHO method were significantly lower in specimens prepared by SpermFertil than in ISolate or Perwash specimens (P < .05). Tail abnormalities were significantly more frequent in specimens prepared by SpermFertil than in ISolate and Perwash specimens (P < .001). Percentage recovery of motile sperm was significantly higher in ISolate and Perwash specimens than in SpermFertil specimens (P < .05). Semen characteristics were similar in specimens prepared with ISolate and with Perwash. At all time intervals, sperm motility was higher in ISolate and Perwash specimens than in SpermFertil specimens (P < .001).Sperm samples separated with a SpermFertil column have poorer sperm quality in several respects than samples prepared with ISolate or Perwash. This finding and the similarity between ISolate and Percoll procedures suggests that ISolate is a good alternative to Percoll-type media to prepare sperm for assisted reproduction.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
SciGPT应助落红禹03采纳,获得10
刚刚
牛马打工人完成签到 ,获得积分20
1秒前
科研小lese完成签到,获得积分10
1秒前
wing完成签到 ,获得积分10
1秒前
鱼0306完成签到,获得积分10
1秒前
金秋完成签到,获得积分0
3秒前
FashionBoy应助头头啊头头啊采纳,获得10
3秒前
miemie66完成签到,获得积分10
4秒前
kakaC完成签到 ,获得积分10
4秒前
萝卜猪完成签到,获得积分10
4秒前
skysleeper完成签到,获得积分10
5秒前
6秒前
yuze完成签到 ,获得积分10
6秒前
hou完成签到 ,获得积分10
7秒前
平淡南霜完成签到,获得积分10
7秒前
摇不滚摇滚完成签到 ,获得积分10
7秒前
8秒前
细心可乐完成签到 ,获得积分10
9秒前
fbwg完成签到 ,获得积分10
9秒前
牛马打工人关注了科研通微信公众号
9秒前
枝桠完成签到,获得积分10
9秒前
浪而而发布了新的文献求助10
11秒前
彩色完成签到,获得积分10
11秒前
孤独梦柏完成签到,获得积分10
13秒前
不想长大完成签到 ,获得积分10
15秒前
黑色幽默完成签到 ,获得积分10
16秒前
桂花完成签到 ,获得积分10
16秒前
17秒前
kobe完成签到,获得积分10
17秒前
英俊的含蕾完成签到 ,获得积分10
21秒前
小绵羊完成签到,获得积分20
21秒前
Vincent完成签到,获得积分10
22秒前
jackie完成签到,获得积分10
23秒前
wwm98656完成签到,获得积分10
23秒前
23秒前
槑槑完成签到 ,获得积分10
23秒前
汉堡包应助默默采纳,获得10
24秒前
taoli完成签到,获得积分20
24秒前
迅速的念芹完成签到 ,获得积分10
25秒前
25秒前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
叶剑英与华南分局档案史料 500
Foreign Policy of the French Second Empire: A Bibliography 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3146969
求助须知:如何正确求助?哪些是违规求助? 2798221
关于积分的说明 7827159
捐赠科研通 2454808
什么是DOI,文献DOI怎么找? 1306480
科研通“疑难数据库(出版商)”最低求助积分说明 627788
版权声明 601565