医学
氧化应激
谷胱甘肽过氧化物酶
丙二醛
超氧化物歧化酶
活性氧
药理学
抗氧化剂
神经保护
三磷酸腺苷
脂质过氧化
生物化学
内科学
化学
作者
Kian Ping Loh,Qi Jia,Benny Kwong Huat Tan,Xin Hua Liu,Bang Guo Wei,Yi Zhun Zhu
出处
期刊:Stroke
[Ovid Technologies (Wolters Kluwer)]
日期:2010-10-15
卷期号:41 (11): 2661-2668
被引量:129
标识
DOI:10.1161/strokeaha.110.589895
摘要
Background and Purpose— Oxidative stress is known to be involved in ischemic stroke. Intense interest is drawn to the therapeutic potential of Chinese herbs on ischemic stroke because many of them contain antioxidant properties. Leonurine, 1 of the active compounds from purified Herba Leonuri, was studied to evaluate its possible therapeutic effects on ischemic stroke. Method— Middle cerebral artery occlusion was selected as our model of study. The animals were pretreated with Leonurine orally for 7 days and the surgery was done. One day after surgery, 2,3,5-triphenyltetrazolium chloride staining and neurological deficit score were carried out to evaluate the functional outcome of animals, whereas levels of superoxide dismutase, glutathione peroxidase, and malondialdehyde were analyzed for oxidative stress analysis. For mitochondrial studies, 3 hours after surgery, mitochondria were isolated for analysis of reactive oxygen species production, adenosine triphosphate biosynthesis, oxygen consumption, and respiratory control ratio value. Result— In in vivo experiments, Leonurine pretreatment reduced infarct volume, improved neurological deficit in stroke groups, increased activities of antioxidant enzymes superoxide dismutase and glutathione peroxidase, and decreased levels from the lipid peroxidation marker malondialdehyde. In terms of mitochondrial modulation, Leonurine inhibited mitochondrial reactive oxygen species production and adenosine triphosphate biosynthesis. Animal studies also demonstrated that the mitochondrial function and redox state were restored by Leonurine treatment. Conclusions— Leonurine has neuroprotective effects and carries a therapeutic potential of stroke prevention.
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