Improved vaccine efficacy of tumor exosome compared to tumor lysate loaded dendritic cells in mice

树突状细胞 免疫疗法 免疫系统 抗原呈递 免疫学 抗原 免疫抑制 CD11c公司 外体 抗原提呈细胞 癌症研究 接种疫苗 免疫监视 T细胞 生物 微泡 表型 小RNA 生物化学 基因
作者
Xiaoyu Gu,Ulrike Erb,Markus W. Büchler,Margot Zöller
出处
期刊:International Journal of Cancer [Wiley]
卷期号:136 (4) 被引量:160
标识
DOI:10.1002/ijc.29100
摘要

Leukemia immunotherapy frequently does not meet expectation, one of the handicaps being tumor exosome (TEX)‐promoted immunosuppression. We here asked, using the mouse myeloid leukemia WEHI3B and the renal cell carcinoma line RENCA, whether dendritic cell (DC) vaccination suffices to counterregulate TEX‐induced immunosuppression and whether TEX could serve as tumor antigen for DC‐loading. DC‐vaccination significantly prolonged the survival time of WEHI3B‐bearing mice, TEX‐loaded DC (DC‐TEX) being superior to lysate‐loaded DC (DC‐lys), even an excess of TEX not interfering with immune response induction. The superior response to DC‐TEX was accompanied by an increase in WEHI3B‐specific CD4 + T cells, evaluated by trogocytosis and proliferation. Similar findings accounted for DC loaded with RENCA TEX. TEX was efficiently taken‐up by DC and TEX uptake supported CD11c, MHCII and IL12 upregulation in DC. Importantly, TEX was partly recruited into the MHCII‐loading compartment such that “TEX” presentation time and recovery in T cells significantly exceeded that of tumor‐lysate. Thus, TEX did not drive DC into a suppressive phenotype and were a superior antigen due to higher efficacy of TEX‐presentation that is supported by prolonged persistence, preferential processing in the MHCII‐loading compartment and pronounced trogocytosis by T helper cells. TEX is present in tumor patients' sera. TEX, recovered and enriched from patients' sera, might well provide an optimized, individual‐specific antigen source for DC‐loading and vaccination.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
3秒前
秀丽白凝完成签到,获得积分20
3秒前
Hululu完成签到 ,获得积分10
4秒前
大智若于完成签到 ,获得积分10
4秒前
HH应助香烟小厨采纳,获得10
4秒前
lhuh完成签到,获得积分20
5秒前
cjj完成签到,获得积分10
7秒前
winterm发布了新的文献求助10
8秒前
taozhiqi完成签到 ,获得积分10
10秒前
冷傲老九完成签到,获得积分10
11秒前
xiangzq完成签到,获得积分10
11秒前
隐形曼青应助隐形之玉采纳,获得10
11秒前
13秒前
0x1orz完成签到,获得积分10
14秒前
研友_Z1x9ln完成签到,获得积分10
17秒前
21秒前
yehaidadao完成签到,获得积分10
22秒前
111发布了新的文献求助10
24秒前
hj456完成签到,获得积分10
25秒前
25秒前
SIRT1发布了新的文献求助10
25秒前
30秒前
泡泡驳回了今后应助
31秒前
NexusExplorer应助SIRT1采纳,获得10
32秒前
0128lun完成签到,获得积分10
32秒前
lhuh发布了新的文献求助10
33秒前
kyt完成签到 ,获得积分10
33秒前
隐形之玉发布了新的文献求助10
34秒前
yaoccccchen完成签到,获得积分10
35秒前
zhaoyang完成签到 ,获得积分10
35秒前
111完成签到,获得积分10
35秒前
慕青应助不许内耗采纳,获得10
40秒前
啦啦啦发布了新的文献求助10
40秒前
感谢有你完成签到 ,获得积分10
41秒前
稳重的山柏完成签到 ,获得积分20
42秒前
42秒前
yif完成签到 ,获得积分10
43秒前
sherry221应助strings采纳,获得20
43秒前
奇奇吃面发布了新的文献求助10
45秒前
高分求助中
Sustainability in Tides Chemistry 2800
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Handbook of Qualitative Cross-Cultural Research Methods 600
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3137627
求助须知:如何正确求助?哪些是违规求助? 2788531
关于积分的说明 7787471
捐赠科研通 2444861
什么是DOI,文献DOI怎么找? 1300119
科研通“疑难数据库(出版商)”最低求助积分说明 625814
版权声明 601023