核酸
化学
检出限
量油尺
核酸检测
萃取(化学)
色谱法
灵敏度(控制系统)
溶解
纳米技术
生物化学
尿
材料科学
电子工程
工程类
作者
Gaihua Cao,Yifan Xiong,Yefeng Qiu,Juntao Yang,Yu Wang,Fuping Nie,Danqun Huo,Changjun Hou
标识
DOI:10.1016/j.aca.2023.341865
摘要
The polymeric chain reaction (PCR) has come under fire for being time-consuming, requiring expensive equipments, and requiring the extraction and purification of nucleic acids. Here, an ultra-fast and sensitive detection platform without nucleic acid extraction solved the above problems. Firstly, the RoomTemp Sample Lysis Kit released the nucleic acid in 3 min and removed the inhibition to facilitate the amplification reaction. What's more, ultra-fast PCR (UF-PCR) can complete 40 cycles in just 15 min and 50 s. To improve the sensitivity and provide more convenient reading modes, CRISPR/Cas12a was mediated to detect Lumpy skin disease virus (LSDV). The platform output fluorescence and Lateral flow dipstick (LFD) signals. The actual detection limit was 2 × 101 copies·μL-1. The portable platform realized visualization, excellent sensitivity and quick speed. In summary, the field-friendly testing platform had great potential in practical testing.
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