生物
DNA
凝聚体
串联(数学)
遗传学
基因
分子生物学
基因组
数学
组合数学
作者
Lauren Dickinson,Wenji Yuan,Chantal LeBlanc,Geoffrey Thomson,Siyuan Wang,Yannick Jacob
出处
期刊:Nature plants
[Springer Nature]
日期:2023-08-31
卷期号:9 (9): 1398-1408
被引量:4
标识
DOI:10.1038/s41477-023-01495-w
摘要
Transformation via Agrobacterium tumefaciens is the predominant method used to introduce exogenous DNA into plant genomes1,2. Transfer DNA (T-DNA) originating from Agrobacterium can be integrated as a single copy or in complex concatenated forms3,4, but the mechanisms affecting final T-DNA structure remain unknown. Here we demonstrate that inclusion of retrotransposon (RT)-derived sequences in T-DNA can increase T-DNA copy number by more than 50-fold in Arabidopsis thaliana. These additional T-DNA copies are organized into large concatemers, an effect primarily induced by the long terminal repeats (LTRs) of RTs that can be replicated using non-LTR DNA repeats. We found that T-DNA concatenation is dependent on the activity of the DNA repair proteins MRE11, RAD17 and ATR. Finally, we show that T-DNA concatenation can be used to increase the frequency of targeted mutagenesis and gene targeting. Overall, this work uncovers molecular determinants that modulate T-DNA copy number in Arabidopsis and demonstrates the utility of inducing T-DNA concatenation for plant gene editing. During transformation via Agrobacterium tumefaciens, sequence composition of T-DNA (that is, DNA repeats) affects T-DNA concatenation in Arabidopsis. In addition, loss of specific DNA repair proteins dramatically decreases T-DNA concatenation levels. This study showcases the potential of T-DNA concatenation as a tool to enhance gene editing.
科研通智能强力驱动
Strongly Powered by AbleSci AI