Loss‐of‐function of PGL10 impairs photosynthesis and tolerance to high‐temperature stress in rice

原叶绿素 叶绿体 突变体 生物 光合作用 互补 叶绿素 生物化学 四吡咯 野生型 基因 植物 细胞生物学
作者
Shakeel Ahmad,Javaria Tabassum,Zhonghua Sheng,Yusong Lv,Wei Chen,Aqib Zeb,Na Dong,Umed Ali,Gaoneng Shao,Xiangjin Wei,Shikai Hu,Shaoqing Tang
出处
期刊:Physiologia Plantarum [Wiley]
卷期号:176 (3)
标识
DOI:10.1111/ppl.14369
摘要

Abstract High temperature (HT) affects the production of chlorophyll (Chl) pigment and inhibits cellular processes that impair photosynthesis, and growth and development in plants. However, the molecular mechanisms underlying heat stress in rice are not fully understood yet. In this study, we identified two mutants varying in leaf color from the ethylmethanesulfonate mutant library of indica rice cv . Zhongjiazao‐17, which showed pale‐green leaf color and variegated leaf phenotype under HT conditions. Mut‐map revealed that both mutants were allelic, and their phenotype was controlled by a single recessive gene PALE GREEN LEAF 10 ( PGL10 ) that encodes NADPH:protochlorophyllide oxidoreductase B, which is required for the reduction of protochlorophyllide into chlorophyllide in light‐dependent tetrapyrrole biosynthetic pathway‐based Chl synthesis. Overexpression‐based complementation and CRISPR/Cas9‐based knockout analyses confirmed the results of Mut‐map. Moreover, qRT‐PCR‐based expression analysis of PGL10 showed that it expresses in almost all plant parts with the lowest expression in root, followed by seed, third leaf, and then other green tissues in both mutants, pgl10a and pgl10b . Its protein localizes in chloroplasts, and the first 17 amino acids from N‐terminus are responsible for signals in chloroplasts. Moreover, transcriptome analysis performed under HT conditions revealed that the genes involved in the Chl biosynthesis and degradation, photosynthesis, and reactive oxygen species detoxification were differentially expressed in mutants compared to WT. Thus, these results indicate that PGL10 is required for maintaining chloroplast function and plays an important role in rice adaptation to HT stress conditions by controlling photosynthetic activity.
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