亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Effects of replacing fishmeal with soybean meal on the immune and antioxidant capacity, and intestinal metabolic functions of red swamp crayfish Procambarus clarkii

克氏原螯虾 小龙虾 生物 沼泽 鱼粉 餐食 豆粕 免疫系统 渔业 食品科学 生态学 免疫学 原材料
作者
Minglang Cai,Xuan Qiu,Huajun Zhang,Aimin Wang,Wen‐Cheng Xu,Kaijian Chen,Zhao He,Yi Hu
出处
期刊:Fish & Shellfish Immunology [Elsevier]
卷期号:149: 109600-109600
标识
DOI:10.1016/j.fsi.2024.109600
摘要

Excess utilization of plant protein sources in animal feed has been found to adversely affect the antioxidant properties and immunity of animals. While the role of gut microbes in plant protein-induced inflammation has been identified in various models, the specific mechanisms regulating gut microbes in crustaceans remain unclear. Accordingly, this study was designed to investigate the effects of replacing fishmeal with soybean meal (SM) on the hepatopancreas antioxidant and immune capacities, and gut microbial functions of crayfish, as well as the potential microbial regulatory mechanisms. 750 crayfish (4.00 g) were randomly divided into five groups: SS0, SS25, SS50, SS75, and SS100, and fed diets with different levels of soybean meal substituted for fishmeal for six weeks. High SM supplementation proved detrimental to maintaining hepatopancreas health, as indicated by an increase in hemolymph MDA content, GPT, and GOT activities, the observed rupture of hepatopancreas cell basement membranes, along with the decreased number of hepatopancreatic F cells. Moreover, crayfish subjected to high SM diets experienced obvious inflammation in hepatopancreas, together with up-regulated mRNA expression levels of nfkb, alf, and tlr (p<0.05), whereas the lzm mRNA expression level exhibited the highest value in the SS25 group. Furthermore, hepatopancreas antioxidant properties highly attenuated by the level of dietary SM substitution levels, as evidenced by the observed increase in MDA content (p<0.05), decrease in GSH content (p<0.05), and inhabitation of SOD, CAT, GPx, and GST activities (p<0.05), along with down-regulated hepatopancreas cat, gpx, gst, and mmnsod mRNA expression levels via inhibiting nrf2/keap1 pathway. Functional genes contributing to metabolism identified that high SM diets feeding significantly activated lipopolysaccharide biosynthesis, revealing gut dysfunction acted as the cause of inflammation. The global microbial co-occurrence network further indicated that the microbes contributing more to serum indicators and immunity were in module eigengene 17 (ME17). A structural equation model revealed that the genes related to alf directly drove the serum enzyme activities through microbes in ME17, with OTU399 and OTU533 identified as major biomarkers and classified into Proteobacteria that secrete endotoxins. To conclude, SM could replace 25% of fishmeal in crayfish diets without negatively affecting immunity, and antioxidant capacity. Excessive SM levels contributed to gut dysfunction and weakened the innate immune system of crayfish.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI

祝大家在新的一年里科研腾飞
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
3秒前
Cindy发布了新的文献求助10
8秒前
Jokic完成签到,获得积分10
20秒前
香蕉觅云应助混子王采纳,获得10
24秒前
30秒前
Cindy完成签到,获得积分20
32秒前
江上游完成签到 ,获得积分10
35秒前
混子王发布了新的文献求助10
36秒前
混子王完成签到,获得积分10
41秒前
1分钟前
liu发布了新的文献求助10
1分钟前
黄花菜完成签到 ,获得积分0
1分钟前
XIE完成签到,获得积分10
1分钟前
1分钟前
orixero应助wanghaiyang采纳,获得10
1分钟前
yuaner发布了新的文献求助10
1分钟前
yuaner完成签到,获得积分10
1分钟前
2分钟前
2分钟前
wanghaiyang发布了新的文献求助10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
Saven完成签到,获得积分10
2分钟前
肥皂完成签到,获得积分10
2分钟前
琛哥物理完成签到,获得积分10
2分钟前
2分钟前
暖暖发布了新的文献求助10
3分钟前
时尚的梦曼完成签到,获得积分10
3分钟前
hty完成签到 ,获得积分10
3分钟前
在水一方应助暖暖采纳,获得10
3分钟前
xuetianshilily完成签到,获得积分10
3分钟前
yema完成签到 ,获得积分10
3分钟前
3分钟前
4分钟前
liqiqi发布了新的文献求助10
4分钟前
4分钟前
科研通AI2S应助科研通管家采纳,获得10
4分钟前
4分钟前
我是老大应助dll采纳,获得10
4分钟前
完美世界应助小满采纳,获得10
4分钟前
4分钟前
高分求助中
Востребованный временем 2500
诺贝尔奖与生命科学 1000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 1000
Kidney Transplantation: Principles and Practice 1000
Separation and Purification of Oligochitosan Based on Precipitation with Bis(2-ethylhexyl) Phosphate Anion, Re-Dissolution, and Re-Precipitation as the Hydrochloride Salt 500
effects of intravenous lidocaine on postoperative pain and gastrointestinal function recovery following gastrointestinal surgery: a meta-analysis 400
The Collected Works of Jeremy Bentham: Rights, Representation, and Reform: Nonsense upon Stilts and Other Writings on the French Revolution 320
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3379091
求助须知:如何正确求助?哪些是违规求助? 2994571
关于积分的说明 8759795
捐赠科研通 2679155
什么是DOI,文献DOI怎么找? 1467494
科研通“疑难数据库(出版商)”最低求助积分说明 678702
邀请新用户注册赠送积分活动 670381