Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora

生物 孢子萌发 转录因子 细胞生物学 分生孢子 转录组 小桶 基因 代谢途径 表型 信号转导 突变体 生物化学 基因表达 微生物学 孢子
作者
Qianqian Liu,Kexin Jiang,Shipeng Duan,Na Zhao,Yanmei Shen,Lirong Zhu,Ke‐Qin Zhang,Jinkui Yang
出处
期刊:Journal of Advanced Research [Elsevier]
被引量:4
标识
DOI:10.1016/j.jare.2024.02.002
摘要

Arthrobotrys oligospora has been utilized as a model strain to study the interaction between fungi and nematodes owing to its ability to capture nematodes by developing specialized traps. A previous study showed that high-osmolarity glycerol (Hog1) signaling regulates the osmoregulation and nematocidal activity of A. oligospora. However, the function of downstream transcription factors of the Hog1 signaling in the nematode-trapping (NT) fungi remains unclear. This study aimed to investigate the functions and potential regulatory network of AoMsn2, a downstream transcription factor of the Hog1 signaling pathway in A. oligospora. The function of AoMsn2 was characterized using targeted gene deletion, phenotypic experiments, real-time quantitative PCR, RNA sequencing, untargeted metabolomics, and yeast two-hybrid analysis. Loss of Aomsn2 significantly enlarged and swollen the hyphae, with an increase in septa and a significant decrease in nuclei. In particular, spore yield, spore germination rate, traps, and nematode predation efficiency were remarkably decreased in the mutants. Phenotypic and transcriptomic analyses revealed that AoMsn2 is essential for fatty acid metabolism and autophagic pathways. Additionally, untargeted metabolomic analysis identified an important function of AoMsn2 in the modulation of secondary metabolites. Furtherly, we analyzed the protein interaction network of AoMsn2 based on the Kyoto Encyclopedia of Genes and Genomes pathway map and the online website STRING. Finally, Hog1 and six putative targeted proteins of AoMsn2 were identified by Y2H analysis. Our study reveals that AoMsn2 plays crucial roles in the growth, conidiation, trap development, fatty acid metabolism, and secondary metabolism, as well as establishes a broad basis for understanding the regulatory mechanisms of trap morphogenesis and environmental adaptation in NT fungi.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
星辰大海应助lilililili采纳,获得10
1秒前
小蘑菇应助科研通管家采纳,获得10
2秒前
情怀应助科研通管家采纳,获得10
2秒前
北长雨安发布了新的文献求助10
2秒前
华仔应助科研通管家采纳,获得10
2秒前
afaf应助科研通管家采纳,获得60
2秒前
丘比特应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
情怀应助科研通管家采纳,获得10
2秒前
梁大海发布了新的文献求助10
3秒前
LJYWJYR完成签到,获得积分10
3秒前
3秒前
大模型应助呆萌的代芹采纳,获得10
3秒前
3秒前
经又夏完成签到,获得积分10
4秒前
4秒前
XiaoMing完成签到,获得积分10
4秒前
5秒前
宇宇关注了科研通微信公众号
5秒前
天下一番发布了新的文献求助10
5秒前
6秒前
CryBill完成签到,获得积分10
6秒前
Akim应助自由的蛋挞采纳,获得10
7秒前
7秒前
455完成签到,获得积分20
7秒前
aaabbb完成签到,获得积分10
7秒前
情怀应助粉色小妖精采纳,获得20
7秒前
无情的夜阑给无情的夜阑的求助进行了留言
8秒前
AoAoo发布了新的文献求助10
8秒前
阿峰发布了新的文献求助10
8秒前
梁大海完成签到,获得积分10
8秒前
12345完成签到,获得积分10
8秒前
9秒前
1111111111发布了新的文献求助10
9秒前
Maggie发布了新的文献求助10
10秒前
阿大发布了新的文献求助10
10秒前
10秒前
11秒前
科研顺利完成签到 ,获得积分10
11秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
The Data Economy: Tools and Applications 1000
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufen 700
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
Ethnicities: Media, Health, and Coping 700
书名《抗体药物研发》 560
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3092869
求助须知:如何正确求助?哪些是违规求助? 2744826
关于积分的说明 7582457
捐赠科研通 2396101
什么是DOI,文献DOI怎么找? 1270838
科研通“疑难数据库(出版商)”最低求助积分说明 614964
版权声明 598844