适体
细胞外小泡
生物传感器
液体活检
荧光
检出限
化学
纳米技术
灵敏度(控制系统)
癌症
癌症研究
细胞生物学
分子生物学
材料科学
生物
色谱法
量子力学
物理
遗传学
电子工程
工程类
作者
Fangfang Guo,Mengxu Sun,Ya Zhang,Juan Xie,Qingxin Gao,Wen‐Jun Duan,Jin-Xiang Chen,Jun Chen,Zong Dai,Minmin Li
标识
DOI:10.1016/j.snb.2023.133890
摘要
Cancer-derived extracellular vesicles (EVs) play a crucial role in intercellular information transmission, tumor metastasis and invasion, which has become one of the most promising biomarkers for tumor liquid biopsy. Herein, we propose a novel dual aptamer recognition-based fluorescent biosensor assisted by functionalized metal−organic frameworks (MOFs) and rolling circle amplification (RCA) method for ultrasensitive cancer-derived EVs assay. This approach exhibits excellent specificity and sensitivity toward EVs. The linear range of EVs detection was 5 × 104 particles/μL to 1 × 107 particles/μL and the limit of detection (LOD) was 2.2 × 104 particles/μL, which is superior to those of most reported methods. Moreover, double aptamer recognition improved the accuracy of this strategy in practical application. This method has been successfully used for the diagnosis of leukemia by detecting EVs from the normal and leukemia patient's serum, which is expected to be applied as an effective tool for EVs research and has considerable potential for early clinical diagnosis of disease.
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