The main factors affecting the water level of Poyang Lake include the inflow, outflow, precipitation and flow backward, etc. The Hydrological Alteration Diagnose System was used to analyze the water level series of Hukou Station to uncover the alteration point, and the alteration point was taken as the basis to divide the time series as before the alteration point and after the alteration point, in order to study the alteration regulation of flow backward. The results show that, compared with the flow backward series before the alteration point, the water level and discharge of flow backward after the alteration point were much more intensity, and the tendency of flow backward discharge was decreasing, the times and lasting period of flow backward were much more reduced at the same time. The regulation appeared above may be one of the reasons that cause the water level decreasing, and dry season advancing of Poyang Lake.