化学
粘液
支气管肺泡灌洗
柠檬酸
豚鼠
绿原酸
汤剂
高碘酸-席夫染色
肺
葡聚糖
药理学
传统医学
染色
色谱法
医学
生物化学
病理
酶
内科学
生物
生态学
作者
Juan Xiang,Xiaodong Liu,Shan Zhong,Zhangfu Fang,Shuirong Shen,Jiaman Tang,Siqi Lai,Kefang Lai
出处
期刊:Journal of Medicinal Food
[Mary Ann Liebert]
日期:2020-02-01
卷期号:23 (2): 191-197
被引量:12
标识
DOI:10.1089/jmf.2019.4423
摘要
Fructus mume was recorded in the Chinese Pharmacopoeia and traditional Chinese medical books for chronic cough, but the effect and related constituents are still unknown. Thus, we investigated the protect effects and the relevant constituents of F. mume in a guinea pig model with chronic cough induced by cigarette smoke (CS). The organic acids and polysaccharides in F. mume were detected by high performance liquid chromatography, gel permeation chromatography, and gas chromatography–mass spectrometry. The guinea pigs were orally administered with vehicle or the water extract of Fructus mume (FW) during the 14 days of CS exposure. Citric acid induced coughs were automatically measured by Buxco system. The differential cells in bronchoalveolar lavage fluid (BALF) and histopathological changes in lung tissue were assessed by hematoxylin and eosin staining. The tumor necrosis factor alpha (TNF-α) and interleukin-8 (IL-8) levels in lung tissue were detected via enzyme-linked immunosorbent assay. The mucus productions in tracheas were determined with Alcian blue-periodic acid Schiff staining. The results suggested relatively high concentration of citric acid, chlorogenic acid, and neochlorogenic acid in F. mume, and high proportion of galactose and glucose and lower molecular weight of polysaccharides. Administration of FW significantly reduced the cough frequency, decreased inflammatory cells in BALF and lung tissue, and attenuated the thickening of airway epithelium and submucosa compared with CS-exposure group. Moreover, the overproduction of TNF-α and IL-8 in lung tissues, and mucus in central airways of CS-induced guinea pigs was markedly inhibited by FW. The extract could also protect against CS exposure-induced chronic cough in guinea pigs by reducing coughs, airway inflammation, and mucus overproduction.
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