清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

A Modified Ultra-Sensitive ELISA for Measurement of LH in Mice

内分泌学 内科学 医学 化学 生物
作者
Michael J Kreisman,Richard B. McCosh,Kellie M. Breen
出处
期刊:Endocrinology [Oxford University Press]
卷期号:163 (9) 被引量:11
标识
DOI:10.1210/endocr/bqac109
摘要

Abstract A major obstacle to monitoring pulsatile luteinizing hormone (LH) secretion in mice has been an assay with sufficient sensitivity in small blood volumes. In 2013, Steyn and colleagues published a highly sensitive enzyme-linked immunosorbent assay (ELISA) that overcame this barrier by coupling a duo of LH antibodies effective in accurately measuring LH in 4-µL whole-blood aliquots. To address the unavailability of the original detection antibody, AFP240580Rb, we validated a replacement detection antibody, biotinylated-5303 SPRN-5, to be used within the established ELISA. This modified LH ELISA demonstrated a minimum detection limit of 0.0028 ng/mL and a limit of quantification of 0.0333 ng/mL or 0.0666 ng/mL in diluted whole-blood samples of volume 6.4 µL (1:10) or 3.2 µL (1:20), respectively. Detection antibody 5303 SPRN-5 demonstrated parallelism, high precision, and accuracy across the standard curve. LH concentrations in comparison assays, using either 5303 SPRN-5 or AFP240580Rb, were highly correlated (R2 = 0.9829) and demonstrated LH pulse profiles from gonadectomized mice that were nearly superimposable. Pulsatile LH secretion was demonstrated in gonad-intact males and diestrous females and basal LH levels measured with 5303 SPRN-5 were approximately 5-fold higher than the limit of quantification. In addition, we document utility of this new LH ELISA to accurately measure LH in whole blood or serum across multiple sampling sites, as well as in pituitary extracts, LβT2 cells, or media. In summary, the modified LH ELISA described here is highly effective in measuring LH across a range of sample types and small volumes in mice.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
8秒前
10秒前
郜郜嗳发布了新的文献求助10
13秒前
怪杰发布了新的文献求助10
14秒前
火星的雪完成签到 ,获得积分10
19秒前
郜郜嗳完成签到,获得积分10
29秒前
万能图书馆应助怪杰采纳,获得10
43秒前
54秒前
54秒前
57秒前
kokoko完成签到,获得积分10
1分钟前
1分钟前
chcmy完成签到 ,获得积分0
1分钟前
量子星尘发布了新的文献求助10
1分钟前
2分钟前
Sunny完成签到,获得积分10
2分钟前
2分钟前
英喆完成签到 ,获得积分10
2分钟前
arsenal完成签到 ,获得积分10
2分钟前
ryan1300完成签到 ,获得积分10
2分钟前
2分钟前
2分钟前
怪杰发布了新的文献求助10
2分钟前
火之高兴完成签到 ,获得积分10
2分钟前
2分钟前
Skywings完成签到,获得积分10
3分钟前
怪杰发布了新的文献求助10
3分钟前
3分钟前
Angela发布了新的文献求助10
3分钟前
JamesPei应助怪杰采纳,获得10
3分钟前
wujiwuhui完成签到 ,获得积分10
3分钟前
量子星尘发布了新的文献求助10
3分钟前
kysl完成签到,获得积分10
3分钟前
Angela完成签到,获得积分10
4分钟前
cgs完成签到 ,获得积分10
4分钟前
iorpi完成签到,获得积分10
4分钟前
kenchilie完成签到 ,获得积分10
4分钟前
4分钟前
无悔完成签到 ,获得积分10
5分钟前
量子星尘发布了新的文献求助10
5分钟前
高分求助中
The Mother of All Tableaux Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 2400
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
Optimal Transport: A Comprehensive Introduction to Modeling, Analysis, Simulation, Applications 800
Official Methods of Analysis of AOAC INTERNATIONAL 600
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
T/CIET 1202-2025 可吸收再生氧化纤维素止血材料 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3957101
求助须知:如何正确求助?哪些是违规求助? 3503095
关于积分的说明 11111294
捐赠科研通 3234212
什么是DOI,文献DOI怎么找? 1787789
邀请新用户注册赠送积分活动 870772
科研通“疑难数据库(出版商)”最低求助积分说明 802292