化学
不稳定性
超氧化物歧化酶
细胞内
金属
二价
超氧化物
配体(生物化学)
咪唑
锰
质谱法
歧化酶
水溶液中的金属离子
生物物理学
抗氧化剂
酶
生物化学
受体
色谱法
有机化学
生物
作者
Martha Zoumpoulaki,Gabrielle Schanne,Nicolas Delsuc,Hugues Preud’homme,Elodie Quévrain,Nicolas Eskenazi,Géraldine Gazzah,Régis Guillot,Philippe Seksik,Joëlle Vinh,Ryszard Łobiński,Clotilde Policar
标识
DOI:10.1002/anie.202203066
摘要
The detection and quantification of exogenous metal complexes are crucial to understanding their activity in intricate biological media. MnII complexes are difficult to detect and quantify because of low association constants, and high lability. The superoxide dismutase (SOD) mimic (or mimetic) labelled Mn1 is based on a 1,2-di-aminoethane functionalized with imidazole and phenolate and has good intrinsic anti-superoxide, antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-activated intestinal epithelial HT29-MD2 cells, similar to that of its propylated analogue labelled Mn1P. Ion mobility spectrometry-mass spectrometry (IMS-MS) is a powerful technique for separating low molecular weight (LMW) metal complexes and can even separate complexes with the same ligand but bound to different divalent metal cations with similar ionic radii. We demonstrated the intracellular presence of the Mn1 and Mn1P complexes, at least partly intact, in lysates of cells incubated with the complexes and estimated the intracellular Mn1P concentration using a Co-13 C6 analogue.
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