表面等离子共振
生物传感器
材料科学
折射率
链霉亲和素
石墨烯
波导管
基质(水族馆)
表面等离子体子
光学
光电子学
分析化学(期刊)
等离子体子
纳米技术
化学
纳米颗粒
色谱法
生物素
生物化学
海洋学
物理
地质学
作者
Chien‐Hsing Chen,Chang-Yue Chiang
出处
期刊:Nanomaterials
[MDPI AG]
日期:2022-06-22
卷期号:12 (13): 2146-2146
被引量:3
摘要
This study develops a highly sensitive and low-cost carboxyl-graphene-oxide-based planar optical waveguide localized surface plasmon resonance biosensor (GO-OW LSPR biosensor), a system based on measuring light intensity changes. The structure of the sensing chip comprises an optical waveguide (OW)-slide glass and microfluidic-poly (methyl methacrylate) (PMMA) substrate, and the OW-slide glass surface-modified gold nanoparticle (AuNP) combined with graphene oxide (GO). As the GO has an abundant carboxyl group (-COOH), the number of capture molecules can be increased. The refractive index sensing system uses silver-coated reflective film to compare the refractive index sensitivity of the GO-OW LSPR biosensor to increase the refractive index sensitivity. The result shows that the signal variation of the system with the silver-coated reflective film is 1.57 times that of the system without the silver-coated reflective film. The refractive index sensitivity is 5.48 RIU-1 and the sensor resolution is 2.52 ± 0.23 × 10-6 RIU. The biochemical sensing experiment performs immunoglobulin G (IgG) and streptavidin detection. The limits of detection of the sensor for IgG and streptavidin are calculated to be 23.41 ± 1.54 pg/mL and 5.18 ± 0.50 pg/mL, respectively. The coefficient of variation (CV) of the repeatability experiment (sample numbers = 3) is smaller than 10.6%. In addition, the affinity constants of the sensor for anti-IgG/IgG and biotin/streptavidin are estimated to be 1.06 × 107 M-1 and 7.30 × 109 M-1, respectively. The result shows that the GO-OW LSPR biosensor has good repeatability and very low detection sensitivity. It can be used for detecting low concentrations or small biomolecules in the future.
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