Alteration of bacterial communities and co-occurrence networks as a legacy effect upon exposure to polyethylene residues under field environment

The use of plastic film mulch threatens the sustainability of the terrestrial environment because of the persistence of plastic residue. Identification of the potential long-term impacts of polyethylene (PE) residue on the soil microbiome has been overlooked in most studies. A long-term field experiment was conducted to expand this understanding by performing a co-occurrence network analysis of bacterial communities among different compartment niches (i.e. plastisphere, rhizosphere, and bulk soil) and three PE residue concentrations to determine the differential operational taxonomic units (OTUs) and keystone taxa. The specific set of bacterial microbes in the plastisphere was different from that of bulk soil and rhizosphere (R2 = 0.372, P < 0.001, PERMANOVA). Totally, 215 and 257 differential OTUs were identified in response to the different compartment niches and PE residue concentrations, respectively. Among these, several hubs or keystone taxa responsible for the exposure to PE residues were further identified, most of which have potential biodegradation functions. Exposure to PE residues led to a reduced network complexity and microbiome stability in the soil ecosystem. This study provides a comprehensive evidence on the alteration of bacterial communities and co-occurrence networks in the terrestrial environment as a legacy effect when exposed to PE residues, and has potential implications for predicting the ecological functions of the soil ecosystem.