Automated Colorimetric Method for Estimating Serum Triglycerides

Abstract A colorimetric adaptation of the fluorometric method of Noble and Campbell is described. Decreased sensitivity, caused by substituting colorimetry for fluorometry, has been improved by increasing the proportion of serum to isopropanol extraction fluid from 1 in 21 to 1 in 11, and by placing an adjustable range expander between colorimeter and recorder. This accurate and reliable adaptation has obviated the need for an expensive fluorometer.