化学
介孔材料
介孔二氧化硅
化学工程
催化作用
吸附
形态学(生物学)
纳米颗粒
透射电子显微镜
吸附剂
水解
纳米技术
有机化学
材料科学
工程类
生物
遗传学
作者
Valeria Califano,A. Costantini,Brigida Silvestri,Virginia Venezia,Stefano Cimino,Filomena Sannino
标识
DOI:10.1515/pac-2018-1202
摘要
Abstract β-Glucosidase (BG) was immobilized by adsorption on wrinkled silica nanoparticles (WSNs) and on tannic acid-templated mesoporous silica nanoparticles (TA-MSNPs). The effect induced by a different morphology of the pores of the sorbent on the catalytic performance of β-glucosidase was investigated. A complete textural and morphological characterization of the two samples was performed by Brunauer–Emmett–Teller (BET) method, Fourier Transform Infrared (FT-IR) and transmission electron microscopy (TEM). The results demonstrated that the catalytic performance of the immobilized enzyme depends on the pores size of sorbent but a key factor is the pores morphology. In fact, the BG immobilized on WSNs and TA-MSNPs (BG/WSNs and BG/TA-MSNPs) shows in both cases good catalytic performances in cellobiose hydrolysis, but the catalyst with the best performance is BG/WSNs, in which the support exhibits a central-radial pore structure and a hierarchical trimodal micro-mesoporous pore size. This peculiar morphology allows the enzyme to settle in a place where the interactions with the walls are maximized, increasing its conformational rigidity. Furthermore, the enzyme is prevalently collocated in the interior of pore so that the pores are not completely capped.
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