Strain Selection and Optimization of Mixed Culture Conditions for Lactobacillus pentosus K1-23 with Antibacterial Activity and Aureobasidium pullulans NRRL 58012 Producing Immune-Enhancing ��-Glucan

短梗霉 微生物学 食品科学 乳酸菌 生物 产气荚膜梭菌 乳酸 单核细胞增生李斯特菌 细菌 发酵 化学 遗传学
作者
Ashokkumar Sekar,Myoungjin Kim,Hyeong Chul Jeong,Keun Kim
出处
期刊:Journal of Microbiology and Biotechnology [Springer Science+Business Media]
卷期号:28 (5): 697-706 被引量:7
标识
DOI:10.4014/jmb.1801.01052
摘要

Lactobacillus pentosus K1-23 was selected from among 25 lactic acid bacterial strains owing to its high inhibitory activity against several pathogenic bacteria, including Escherichia coli, Salmonella typhimurium, S. gallinarum, Staphylococcus aureus, Pseudomonas aeruginosa, Clostridium perfringens, and Listeria monocytogenes. Additionally, among 13 strains of Aureobasidium spp., A. pullulans NRRL 58012 was shown to produce the highest amount of β-glucan (15.45 ± 0.07%) and was selected. Next, the optimal conditions for a solid-phase mixed culture with these two different microorganisms (one bacterium and one yeast) were determined. The optimal inoculum sizes for L. pentosus and A. pullulans were 1% and 5%, respectively. The appropriate inoculation time for L. pentosus K1-23 was 3 days after the inoculation of A. pullulans to initiate fermentation. The addition of 0.5% corn steep powder and 0.1% FeSO₄ to the basal medium resulted in the increased production of lactic acid bacterial cells and β-glucan. The following optimal conditions for solid-phase mixed culture were also statistically determined by using the response surface method: 37.84°C, pH 5.25, moisture content of 60.82%, and culture time of 6.08 days for L. pentosus; and 24.11°C, pH 5.65, moisture content of 60.08%, and culture time of 5.71 days for A. pullulans. Using the predicted optimal conditions, the experimental production values of L. pentosus cells and β-glucan were 3.15 ± 0.10 × 10⁸ CFU/g and 13.41 ± 0.04%, respectively. This mixed culture may function as a highly efficient antibiotic substitute based on the combined action of its anti-pathogenic bacterial and immune-enhancing activities.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
李欣月完成签到,获得积分10
刚刚
1秒前
2秒前
2秒前
NexusExplorer应助大蒜头采纳,获得10
2秒前
小马甲应助梦溪采纳,获得10
3秒前
4秒前
难过千易发布了新的文献求助10
4秒前
4秒前
尊敬飞鸟完成签到 ,获得积分10
5秒前
一支布洛芬完成签到,获得积分20
5秒前
6秒前
Phoenix Hu发布了新的文献求助10
7秒前
7秒前
长乐完成签到,获得积分10
7秒前
8秒前
明矾发布了新的文献求助10
8秒前
10秒前
10秒前
10秒前
lll完成签到,获得积分10
11秒前
11秒前
12秒前
整齐的忆彤完成签到,获得积分10
13秒前
伊斯坦布尔的鱼应助叁肆采纳,获得10
15秒前
akjsi发布了新的文献求助10
15秒前
毛毛发布了新的文献求助10
15秒前
16秒前
16秒前
顺心的笑翠完成签到 ,获得积分10
17秒前
17秒前
科研通AI2S应助zy采纳,获得10
18秒前
思源应助晚来天欲雪采纳,获得10
18秒前
19秒前
思源应助酷酷的水儿采纳,获得10
20秒前
桂桂发布了新的文献求助10
20秒前
量子星尘发布了新的文献求助10
20秒前
21秒前
乐乐应助坦率的大神采纳,获得10
26秒前
drtianyunhong完成签到,获得积分10
27秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976177
求助须知:如何正确求助?哪些是违规求助? 3520366
关于积分的说明 11202970
捐赠科研通 3256899
什么是DOI,文献DOI怎么找? 1798535
邀请新用户注册赠送积分活动 877725
科研通“疑难数据库(出版商)”最低求助积分说明 806516