麦角甾醇
角鲨烯
甾醇
还原酶
角鲨烯单加氧酶
酵母
生物化学
酿酒酵母
酶
辅酶A
胞浆
7-脱氢胆固醇还原酶
生物
甲戊酸
HMG-CoA还原酶
甾醇调节元件结合蛋白
羟甲基戊二酰辅酶A还原酶
胆固醇
生物合成
作者
Thomas Polakowski,Ulf Ståhl,Christine Lang
标识
DOI:10.1007/s002530051138
摘要
The enzyme 3-hydroxy-3-methylglutaryl-co-enzyme-A (HMG-CoA) reductase is known as the rate limiting enzyme in early sterol biosynthesis in eukaryotic cells. To eliminate this regulation in the yeast Saccharomyces cerevisiae, a truncated HMG1 gene, producing a form of the enzyme that lacks the membrane-binding region (i.e. amino acids 1-552), was constructed and overexpressed in this yeast. The transformed strains accumulated large amounts of the sterol precursor squalene, while the levels of ergosterol and a number of other sterol compounds were only slightly elevated. These findings suggest that HMG-CoA reductase is not the only rate-limiting step in sterol synthesis and its overexpression cannot significantly influence this pathway beyond the sterol precursor squalene.
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