祖细胞
干细胞因子
巨核细胞
克隆形成试验
川地34
白细胞介素3
分子生物学
干细胞
生物
促红细胞生成素
化学
生长因子
细胞生物学
男科
免疫学
内分泌学
生物化学
细胞凋亡
受体
医学
T细胞
抗原提呈细胞
免疫系统
作者
Jamie Case,Christine Hicks,Annette Trickett,Y. L. Kwan,A. Manoharan
出处
期刊:Journal of Interferon and Cytokine Research
[Mary Ann Liebert]
日期:2006-02-01
卷期号:26 (2): 76-82
被引量:7
标识
DOI:10.1089/jir.2006.26.76
摘要
This study aimed to determine the optimal growth factor combination for expansion of megakaryocyte (Mk) progenitors with clonogenic potential from CD34+-enriched mobilized peripheral blood stem cells (PBSC). Mobilized PBSC were monocyte depleted and CD34+ enriched, then cultured with various combinations of interleukin-3 (IL-3), IL-6, IL-11, Flt3 ligand (Flt3-L), stem cell factor (SCF), granulocyte-macrophage colonystimulating factor (GM-CSF), and erythropoietin (EPO), using a 2(7-3) IV fractional factorial design. Expansion of Mk committed progenitors (CD41+) and primitive precursors (CD61+ CD34+) was determined using FACS and colony-forming assays. Amplification of Mk progenitor production was attributed to IL-3 (p < 0.002), SCF (p < 0.001), and GM-CSF (p < 0.05). Flt3-L inhibited the production of total CD61+ cells (p < 0.05), CD61+CD34+ cells (p < 0.03), and total CD41a+ cells (p < 0.01). Addition of Flt3-L to the optimum growth factor combination of megakaryocyte growth and development factor (MGDF), SCF, IL-3, and GM-CSF caused the greatest increase in total nucleated cells but reduced Mk progenitor expansion. There was also a 20% reduction in Mk+ colonies from cells expanded in the presence of Flt3-L. Factorial analysis identified the optimal combination of growth factors required to expand Mk precursors with clonogenic potential. The addition of Flt3-L to the optimal combination of MGDF, SCF, IL-3, and GM-CSF reduced both the fold expansion of Mk progenitors and Mk colony numbers.
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